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Literature summary extracted from

  • Silver, G.M.; Fall, R.
    Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere (1995), J. Biol. Chem., 270, 13010-13016.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.27 additional information the enzyme is positively regulated by light Populus tremuloides

General Stability

EC Number General Stability Organism
4.2.3.27 glycerol stabilizes the enzyme in the early stages of purification, PEG in the late stages Populus tremuloides

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.27 dimethylallyl diphosphate substrate inhibition above 10 mM Populus tremuloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.27 8
-
 dimethylallyl diphosphate
-
 Populus tremuloides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.27 Mg2+ dependent on Populus tremuloides
4.2.3.27 Mn2+ can substitute for Mg2+ Populus tremuloides
4.2.3.27 additional information the enzyme is strictly dependent on divalent cations, no activity with Zn2+ and Ca2+ Populus tremuloides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.27 58000
-
 1 * 58000 + 1 * 63000, SDS-PAGE Populus tremuloides
4.2.3.27 63000
-
 1 * 58000 + 1 * 63000, SDS-PAGE Populus tremuloides
4.2.3.27 98000 137000 gel filtration Populus tremuloides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Populus tremuloides
-
 isoprene + diphosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Populus tremuloides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.27 native enzyme 4000fold from leaves to homogeneity, ammonium sulfate fractionation of cell-free supernatant, dialysis, ion exchange chromatography, ultrafiltration, and gel filtration Populus tremuloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.27 leaf
-
 Populus tremuloides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.27 0.521
-
 purified enzyme Populus tremuloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
 Populus tremuloides isoprene + diphosphate
-
 ir
4.2.3.27 dimethylallyl diphosphate
-
 Populus tremuloides isoprene + diphosphate product stoichiometry determination, isoprene is volatile 2-methyl-1,3-butadiene emitted by the plant into the atmosphere, where it plays an important role, the enzyme is positively regulated by light ir

Subunits

EC Number Subunits Comment Organism
4.2.3.27 dimer 1 * 58000 + 1 * 63000, SDS-PAGE Populus tremuloides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.27 32
-
 assay at Populus tremuloides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.27 1.7
-
 dimethylallyl diphosphate
-
 Populus tremuloides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.27 8
-
 assay at Populus tremuloides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.3.27 Populus tremuloides isoelectric focusing
-
 4.7