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Literature summary extracted from

  • Myers, R.W.; Wray, J.W.; Fish, S.; Abeles, R.H.
    Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae (1993), J. Biol. Chem., 268, 24785-24791.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.87 overexpression in Klebsiella pneumoniae strain CG253 leading to 5-6fold increased activity compared to the parent strain Klebsiella pneumoniae
5.3.2.5 overexpression in Klebsiella pneumoniae strain CG253 leading to 5-6fold increased activity compared to the parent strain Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.87 additional information no inhibition by phosphate Klebsiella pneumoniae
5.3.2.5 additional information no inhibition by phosphate Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.77 0.0071
-
 2-hydroxy-3-keto-1-phospho-1-hexene
-
 Klebsiella pneumoniae
3.1.3.77 0.077
-
 2,3-Diketo-1-phosphohexane
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.77 Mg2+ required for activity Klebsiella pneumoniae
3.1.3.87 Mg2+ required Klebsiella pneumoniae
5.3.2.5 Mg2+ required Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.77 25500
-
 gel filtration Klebsiella pneumoniae
3.1.3.77 27000
-
 calculated from sequence Klebsiella pneumoniae
3.1.3.77 28300
-
 SDS-PAGE Klebsiella pneumoniae
3.1.3.87 25500
-
 gel filtration Klebsiella pneumoniae
3.1.3.87 28300
-
 1 * 28300, SDS-PAGE Klebsiella pneumoniae
5.3.2.5 25500
-
 gel filtration Klebsiella pneumoniae
5.3.2.5 28300
-
 1 * 28300, SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.87 2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O Klebsiella pneumoniae
-
 1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
 ?
3.1.3.87 2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O Klebsiella pneumoniae CG253
-
 1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
 ?
5.3.2.5 2,3-diketo-1-phosphohexane Klebsiella pneumoniae an alternate substrate for the methionine salvage pathway 2-hydroxy-3-keto-l-phospho-l-hexene
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.77 Klebsiella pneumoniae
-
 CG253
-
3.1.3.77 Klebsiella pneumoniae CG253
-
 CG253
-
3.1.3.87 Klebsiella pneumoniae
-
-
-
3.1.3.87 Klebsiella pneumoniae CG253
-
-
-
5.3.2.5 Klebsiella pneumoniae
-
-
-
5.3.2.5 Klebsiella pneumoniae CG253
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.77 100fold purified to homogeneity in 10% yield, SDS-PAGE, amino acid sequence analysis Klebsiella pneumoniae
3.1.3.87 native enzyme by fractionation with help of protamine suzlfate, ammonium sulfate, and P-60, followed by anion exchange and hydrophobic interaction chromatography Klebsiella pneumoniae
5.3.2.5 native enzyme by fractionation with help of protamine suzlfate, ammonium sulfate, and P-60, followed by anion exchange and hydrophobic interaction chromatography Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.77 21
-
 purified enzyme by phenyl-Superose column Klebsiella pneumoniae
3.1.3.87 additional information
-
 overall pathway activity, purified enzyme, pH 7.5, 30°C Klebsiella pneumoniae
5.3.2.5 additional information
-
 overall pathway activity, purified enzyme, pH 7.5, 30°C Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.77 2,3-diketo-1-phosphohexane + H2O including two intermediates, 2-hydroxy-3-keto-1-phospho-1-hexene and 1,2-dihydroxy-3-keto-1-hexene Klebsiella pneumoniae 2-ketopentanoate + formate + glyoxylate + butyrate
-
 ?
3.1.3.77 2,3-diketo-1-phosphohexane + H2O including two intermediates, 2-hydroxy-3-keto-1-phospho-1-hexene and 1,2-dihydroxy-3-keto-1-hexene Klebsiella pneumoniae CG253 2-ketopentanoate + formate + glyoxylate + butyrate
-
 ?
3.1.3.77 2-hydroxy-3-keto-1-phospho-1-hexene + H2O
-
 Klebsiella pneumoniae 2-ketopentanoate + formate + glyoxylate + butyrate
-
 ?
3.1.3.77 2-hydroxy-3-keto-1-phospho-1-hexene + H2O
-
 Klebsiella pneumoniae CG253 2-ketopentanoate + formate + glyoxylate + butyrate
-
 ?
3.1.3.87 2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
 Klebsiella pneumoniae 1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
 ?
3.1.3.87 2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
 Klebsiella pneumoniae 1,2-dihydroxy-3-oxo-1-hexene + phosphate product determination by NMR analysis. Conversion of 1,2-dihydroxy-3-keto-l-hexene to formate and 2-ketopentanoate occurs non-enzymatically ?
3.1.3.87 2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
 Klebsiella pneumoniae CG253 1,2-dihydroxy-3-oxo-1-hexene + phosphate
-
 ?
3.1.3.87 2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O
-
 Klebsiella pneumoniae CG253 1,2-dihydroxy-3-oxo-1-hexene + phosphate product determination by NMR analysis. Conversion of 1,2-dihydroxy-3-keto-l-hexene to formate and 2-ketopentanoate occurs non-enzymatically ?
3.1.3.87 additional information the phosphatase activity of the enzyme is highly specific, it shows no hydrolysis of phosphoenolpyruvate or 4-nitrophenyl phosphate Klebsiella pneumoniae ?
-
 ?
3.1.3.87 additional information the phosphatase activity of the enzyme is highly specific, it shows no hydrolysis of phosphoenolpyruvate or 4-nitrophenyl phosphate Klebsiella pneumoniae CG253 ?
-
 ?
5.3.2.5 2,3-diketo-1-phosphohexane an alternate substrate for the methionine salvage pathway Klebsiella pneumoniae 2-hydroxy-3-keto-l-phospho-l-hexene
-
 ?
5.3.2.5 2,3-diketo-1-phosphohexane an analogue to 2,3-diketo-5-methylthio-1-phosphopentane Klebsiella pneumoniae 2-hydroxy-3-keto-l-phospho-l-hexene a chromophoric intermediate compound, product determination by NMR analysis ?
5.3.2.5 2,3-diketo-l-phospho-5-thiomethylpentane
-
 Klebsiella pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.77 monomer
-
 Klebsiella pneumoniae
3.1.3.87 monomer 1 * 28300, SDS-PAGE Klebsiella pneumoniae
5.3.2.5 monomer 1 * 28300, SDS-PAGE Klebsiella pneumoniae

Synonyms

EC Number Synonyms Comment Organism
3.1.3.77 E-1
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.87 30
-
 assay at Klebsiella pneumoniae
5.3.2.5 30
-
 assay at Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.87 7.5
-
 assay at Klebsiella pneumoniae
5.3.2.5 7.5
-
 assay at Klebsiella pneumoniae

General Information

EC Number General Information Comment Organism
3.1.3.87 metabolism Klebsiella pneumoniae converts 2,3-dioxo-5-methylthio-1-phosphopentane to formate, 2-oxo-4-methylthiobutyrate, and 3-methylthiopropionate. 2,3-Dioxo-1-phosphohexane, the desthio analogue of the naturally occurring diketone, can serve as alternate substrate in the methionine salvage pathway Klebsiella pneumoniae
5.3.2.5 metabolism Klebsiella pneumoniae converts 2,3-diketo-5-methylthio-l-phosphopentane to formate, 2-keto-4-methylthiobutyrate, and 3-methylthiopropionate. 2,3-diketo-1-phosphohexane, the desthio analogue of the naturally occurring diketone, can serve as alternate substrate in the methionine salvage pathway Klebsiella pneumoniae