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Literature summary extracted from

  • Ishikura, Y.; Tsuzuki, S.; Takahashi, O.; Tokuda, C.; Nakanishi, R.; Shinoda, T.; Taguchi, H.
    Recognition site for the side chain of 2-ketoacid substrate in D-lactate dehydrogenase (2005), J. Biochem., 138, 741-749.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.28 F299G mutation reduces enzyme activity with less marked change in substrate preference Lactiplantibacillus pentosus
1.1.1.28 F299S mutation reduces enzyme activity with less marked change in substrate preference Lactiplantibacillus pentosus
1.1.1.28 Y52A mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate Lactiplantibacillus pentosus
1.1.1.28 Y52D mutation severely reduces enzyme activity Lactiplantibacillus pentosus
1.1.1.28 Y52R mutation severely reduces enzyme activity, preference for large aliphatic 2-ketoacids and phenylpyruvate Lactiplantibacillus pentosus
1.1.1.28 Y52R/F299G mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate and oxaloacetate, weak activity with 2-ketocaproate, 2-ketoglutarate and phenylpyruvate Lactiplantibacillus pentosus
1.1.1.28 Y52T mutation severely reduces enzyme activity Lactiplantibacillus pentosus
1.1.1.28 Y52T/F299S mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate, 2-ketoglutarate and oxaloacetate, weak activity with hydroxypyruvate and phenylpyruvate Lactiplantibacillus pentosus
1.1.1.28 Y52V mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate Lactiplantibacillus pentosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 0.03
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 0.06
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 0.12
-
pyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 0.124
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 0.14
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 0.15
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 0.28
-
Hydroxypyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 0.38
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 0.7
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 0.8
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 0.8
-
phenylpyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 1.18
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
1.1.1.28 1.8
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 2.1
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
1.1.1.28 2.1
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
1.1.1.28 2.2
-
oxaloacetate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 3
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 3.5
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 3.8
-
oxaloacetate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
1.1.1.28 5.5
-
2-ketoisovalerate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 6.9
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
1.1.1.28 7.2
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
1.1.1.28 8.1
-
2-Ketobutyrate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 8.7
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52T/D299S Lactiplantibacillus pentosus
1.1.1.28 8.7
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 11.7
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 12.1
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 12.6
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 13.1
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 13.3
-
2-ketocaproate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 14.1
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
1.1.1.28 15.5
-
2-Ketoglutarate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 17
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 17
-
2-ketovalerate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 18.6
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
1.1.1.28 18.6
-
2-ketoisovalerate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 20.2
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
1.1.1.28 20.8
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 22
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
1.1.1.28 31
-
pyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
1.1.1.28 31
-
2-ketoisocaproate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 43.1
-
oxaloacetate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
1.1.1.28 51
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 53
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
1.1.1.28 102
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 189
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Lactiplantibacillus pentosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 2-ketocaproate + NADH
-
Lactiplantibacillus pentosus 2-hydroxycaproate + NAD+
-
?
1.1.1.28 2-ketoisocaproate + NADH
-
Lactiplantibacillus pentosus 2-hydroxyisocaproate + NAD+
-
?
1.1.1.28 2-ketoisovalerate + NADH
-
Lactiplantibacillus pentosus 2-hydroxyisovalerate + NAD+
-
?
1.1.1.28 2-ketovalerate + NADH
-
Lactiplantibacillus pentosus 2-hydroxyvalerate + NAD+
-
?
1.1.1.28 2-oxobutyrate + NADH + H+
-
Lactiplantibacillus pentosus 2-hydroxybutyrate + NAD+
-
?
1.1.1.28 2-oxoglutarate + NADH + H+
-
Lactiplantibacillus pentosus 2-hydroxypentanedioate + NAD+
-
?
1.1.1.28 hydroxypyruvate + NADH
-
Lactiplantibacillus pentosus glycerate + NAD+
-
?
1.1.1.28 oxaloacetate + NADH
-
Lactiplantibacillus pentosus malate + NAD+
-
?
1.1.1.28 phenylpyruvate + NADH
-
Lactiplantibacillus pentosus phenyllactate + NAD+
-
?
1.1.1.28 pyruvate + NADH + H+
-
Lactiplantibacillus pentosus (R)-lactate + NAD+
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.28 0.056
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 0.3
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 0.31
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
1.1.1.28 0.36
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
1.1.1.28 0.48
-
oxaloacetate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
1.1.1.28 0.51
-
2-Ketoglutarate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 0.7
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 2.1
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
1.1.1.28 2.8
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
1.1.1.28 3.4
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
1.1.1.28 5.2
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52T/D299S Lactiplantibacillus pentosus
1.1.1.28 5.7
-
2-ketovalerate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 6.5
-
oxaloacetate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
1.1.1.28 8.1
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
1.1.1.28 11.1
-
2-ketoisocaproate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 11.7
-
2-ketoisovalerate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 13.8
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
1.1.1.28 15.8
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 15.9
-
2-ketocaproate 30°C, pH 5.5, mutant enzyme Y52R Lactiplantibacillus pentosus
1.1.1.28 17
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 19
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52R/F299G Lactiplantibacillus pentosus
1.1.1.28 21
-
2-ketocaproate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 22
-
pyruvate 30°C, pH 5.5, mutant enzyme F299G Lactiplantibacillus pentosus
1.1.1.28 25.5
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 27
-
2-ketoisovalerate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 35.8
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme F299S Lactiplantibacillus pentosus
1.1.1.28 37
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 40
-
phenylpyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 41
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 41.5
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 50
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 55.7
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 65.7
-
2-ketoisocaproate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 102
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 104
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 118
-
2-Ketobutyrate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 171
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52V Lactiplantibacillus pentosus
1.1.1.28 175
-
2-Ketobutyrate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 213
-
pyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 232
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52A Lactiplantibacillus pentosus
1.1.1.28 257
-
Hydroxypyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 280
-
oxaloacetate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 321
-
pyruvate 30°C, pH 5.5, wild-type enzyme Lactiplantibacillus pentosus
1.1.1.28 334
-
2-ketovalerate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 407
-
Hydroxypyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus
1.1.1.28 778
-
phenylpyruvate 30°C, pH 5.5, mutant enzyme Y52L Lactiplantibacillus pentosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NADH
-
Lactiplantibacillus pentosus