EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.28 | F299G | mutation reduces enzyme activity with less marked change in substrate preference | Lactiplantibacillus pentosus |
1.1.1.28 | F299S | mutation reduces enzyme activity with less marked change in substrate preference | Lactiplantibacillus pentosus |
1.1.1.28 | Y52A | mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate | Lactiplantibacillus pentosus |
1.1.1.28 | Y52D | mutation severely reduces enzyme activity | Lactiplantibacillus pentosus |
1.1.1.28 | Y52R | mutation severely reduces enzyme activity, preference for large aliphatic 2-ketoacids and phenylpyruvate | Lactiplantibacillus pentosus |
1.1.1.28 | Y52R/F299G | mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate and oxaloacetate, weak activity with 2-ketocaproate, 2-ketoglutarate and phenylpyruvate | Lactiplantibacillus pentosus |
1.1.1.28 | Y52T | mutation severely reduces enzyme activity | Lactiplantibacillus pentosus |
1.1.1.28 | Y52T/F299S | mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate, 2-ketoglutarate and oxaloacetate, weak activity with hydroxypyruvate and phenylpyruvate | Lactiplantibacillus pentosus |
1.1.1.28 | Y52V | mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate | Lactiplantibacillus pentosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.28 | 0.03 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.06 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.12 | - |
pyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.124 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.14 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.15 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.28 | - |
Hydroxypyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.38 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.7 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.8 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.8 | - |
phenylpyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 1.18 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
1.1.1.28 | 1.8 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 2.1 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
1.1.1.28 | 2.1 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 2.2 | - |
oxaloacetate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 3 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 3.5 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 3.8 | - |
oxaloacetate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
1.1.1.28 | 5.5 | - |
2-ketoisovalerate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 6.9 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 7.2 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
1.1.1.28 | 8.1 | - |
2-Ketobutyrate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 8.7 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52T/D299S | Lactiplantibacillus pentosus | |
1.1.1.28 | 8.7 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 11.7 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 12.1 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 12.6 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 13.1 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 13.3 | - |
2-ketocaproate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 14.1 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 15.5 | - |
2-Ketoglutarate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 17 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 17 | - |
2-ketovalerate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 18.6 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
1.1.1.28 | 18.6 | - |
2-ketoisovalerate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 20.2 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
1.1.1.28 | 20.8 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 22 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 31 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 31 | - |
2-ketoisocaproate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 43.1 | - |
oxaloacetate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
1.1.1.28 | 51 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 53 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 102 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 189 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.28 | Lactiplantibacillus pentosus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.28 | 2-ketocaproate + NADH | - |
Lactiplantibacillus pentosus | 2-hydroxycaproate + NAD+ | - |
? | |
1.1.1.28 | 2-ketoisocaproate + NADH | - |
Lactiplantibacillus pentosus | 2-hydroxyisocaproate + NAD+ | - |
? | |
1.1.1.28 | 2-ketoisovalerate + NADH | - |
Lactiplantibacillus pentosus | 2-hydroxyisovalerate + NAD+ | - |
? | |
1.1.1.28 | 2-ketovalerate + NADH | - |
Lactiplantibacillus pentosus | 2-hydroxyvalerate + NAD+ | - |
? | |
1.1.1.28 | 2-oxobutyrate + NADH + H+ | - |
Lactiplantibacillus pentosus | 2-hydroxybutyrate + NAD+ | - |
? | |
1.1.1.28 | 2-oxoglutarate + NADH + H+ | - |
Lactiplantibacillus pentosus | 2-hydroxypentanedioate + NAD+ | - |
? | |
1.1.1.28 | hydroxypyruvate + NADH | - |
Lactiplantibacillus pentosus | glycerate + NAD+ | - |
? | |
1.1.1.28 | oxaloacetate + NADH | - |
Lactiplantibacillus pentosus | malate + NAD+ | - |
? | |
1.1.1.28 | phenylpyruvate + NADH | - |
Lactiplantibacillus pentosus | phenyllactate + NAD+ | - |
? | |
1.1.1.28 | pyruvate + NADH + H+ | - |
Lactiplantibacillus pentosus | (R)-lactate + NAD+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.28 | 0.056 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.3 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.31 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.36 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.48 | - |
oxaloacetate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.51 | - |
2-Ketoglutarate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 0.7 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 2.1 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
1.1.1.28 | 2.8 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 3.4 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 5.2 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52T/D299S | Lactiplantibacillus pentosus | |
1.1.1.28 | 5.7 | - |
2-ketovalerate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 6.5 | - |
oxaloacetate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
1.1.1.28 | 8.1 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 11.1 | - |
2-ketoisocaproate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 11.7 | - |
2-ketoisovalerate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 13.8 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
1.1.1.28 | 15.8 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 15.9 | - |
2-ketocaproate | 30°C, pH 5.5, mutant enzyme Y52R | Lactiplantibacillus pentosus | |
1.1.1.28 | 17 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 19 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52R/F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 21 | - |
2-ketocaproate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 22 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme F299G | Lactiplantibacillus pentosus | |
1.1.1.28 | 25.5 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 27 | - |
2-ketoisovalerate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 35.8 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme F299S | Lactiplantibacillus pentosus | |
1.1.1.28 | 37 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 40 | - |
phenylpyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 41 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 41.5 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 50 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 55.7 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 65.7 | - |
2-ketoisocaproate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 102 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 104 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 118 | - |
2-Ketobutyrate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 171 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52V | Lactiplantibacillus pentosus | |
1.1.1.28 | 175 | - |
2-Ketobutyrate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 213 | - |
pyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 232 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52A | Lactiplantibacillus pentosus | |
1.1.1.28 | 257 | - |
Hydroxypyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 280 | - |
oxaloacetate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 321 | - |
pyruvate | 30°C, pH 5.5, wild-type enzyme | Lactiplantibacillus pentosus | |
1.1.1.28 | 334 | - |
2-ketovalerate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 407 | - |
Hydroxypyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus | |
1.1.1.28 | 778 | - |
phenylpyruvate | 30°C, pH 5.5, mutant enzyme Y52L | Lactiplantibacillus pentosus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | NADH | - |
Lactiplantibacillus pentosus |