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Literature summary extracted from

  • Yang, Z.; Lu, C.D.
    Functional genomics enables identification of genes of the arginine transaminase pathway in Pseudomonas aeruginosa (2007), J. Bacteriol., 189, 3945-3953.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.84 L-arginine induces the L-ATase activity at least 76fold in an aruF mutant strain, but not in the wild-type strain PAO1 Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.84 gene aruH or PA4976, location in the aruIH locus Pseudomonas aeruginosa
4.1.1.75 expressed in Escherichia coli DH5alpha cells Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.84 additional information construction of the PA4976 aruH knockout mutant Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.84 additional information Pseudomonas aeruginosa arginine utilization proceeds via multiple catabolic pathways in the organism, the enzyme is involved in the arginine transaminase pathway, regulation of arginine-responsive induction of the arginine transaminase pathway, arginine metabolism, overview ?
-
 ?
2.6.1.84 additional information Pseudomonas aeruginosa knockout of aruH and aruI, which encode an arginine:pyruvate transaminase and a 2-ketoarginine decarboxylase in an operon, also abolishes the ability of the aruF mutant to grow on L-arginine ?
-
 ?
2.6.1.84 additional information Pseudomonas aeruginosa PAO4558 arginine utilization proceeds via multiple catabolic pathways in the organism, the enzyme is involved in the arginine transaminase pathway, regulation of arginine-responsive induction of the arginine transaminase pathway, arginine metabolism, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.84 Pseudomonas aeruginosa
-
-
-
2.6.1.84 Pseudomonas aeruginosa
-
 and several strains derived thereof, gene aruH
-
2.6.1.84 Pseudomonas aeruginosa Q9HUI9
-
-
2.6.1.84 Pseudomonas aeruginosa PAO4558
-
-
-
4.1.1.75 Pseudomonas aeruginosa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.84 L-arginine + pyruvate = 5-guanidino-2-oxopentanoate + L-alanine aruF mutant with inducible L-arginine:pyruvate transaminase activity Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.84 additional information
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.84 L-arginine + pyruvate
-
 Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + L-alanine
-
 ?
2.6.1.84 L-arginine + pyruvate
-
 Pseudomonas aeruginosa PAO4558 5-guanidino-2-oxopentanoate + L-alanine
-
 ?
2.6.1.84 additional information arginine utilization proceeds via multiple catabolic pathways in the organism, the enzyme is involved in the arginine transaminase pathway, regulation of arginine-responsive induction of the arginine transaminase pathway, arginine metabolism, overview Pseudomonas aeruginosa ?
-
 ?
2.6.1.84 additional information AruH is also an L-ATase, overview Pseudomonas aeruginosa ?
-
 ?
2.6.1.84 additional information knockout of aruH and aruI, which encode an arginine:pyruvate transaminase and a 2-ketoarginine decarboxylase in an operon, also abolishes the ability of the aruF mutant to grow on L-arginine Pseudomonas aeruginosa ?
-
 ?
2.6.1.84 additional information arginine utilization proceeds via multiple catabolic pathways in the organism, the enzyme is involved in the arginine transaminase pathway, regulation of arginine-responsive induction of the arginine transaminase pathway, arginine metabolism, overview Pseudomonas aeruginosa PAO4558 ?
-
 ?
2.6.1.84 additional information AruH is also an L-ATase, overview Pseudomonas aeruginosa PAO4558 ?
-
 ?
4.1.1.75 5-Guanidino-2-oxopentanoate
-
 Pseudomonas aeruginosa 4-Guanidinobutyraldehyde + CO2
-
 ?
4.1.1.75 5-Guanidino-2-oxopentanoate
-
 Pseudomonas aeruginosa PAO4558 4-Guanidinobutyraldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.84 arginine:pyruvate transaminase
-
 Pseudomonas aeruginosa
2.6.1.84 aruF mutant
-
 Pseudomonas aeruginosa
2.6.1.84 AruH
-
 Pseudomonas aeruginosa
2.6.1.84 ATASE detected, when pyruvate is used as the amino group acceptor Pseudomonas aeruginosa
2.6.1.84 L-arginine inducible arginine:pyruvate transaminase
-
 Pseudomonas aeruginosa
4.1.1.75 2-KA decarboxylase
-
 Pseudomonas aeruginosa
4.1.1.75 2-ketoarginine decarboxylase
-
 Pseudomonas aeruginosa
4.1.1.75 AruI
-
 Pseudomonas aeruginosa