BRENDA - Enzyme Database show

Identification and characterization of a novel bacterial sulfite oxidase with no heme binding domain from Deinococcus radiodurans

D'Errico, G.; Di Salle, A.; La Cara, F.; Rossi, M.; Cannio, R.; J. Bacteriol. 188, 694-701 (2006)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.3.1
Tris-acetate
up to a concentration of 70 mM, up to 2fold activation
Deinococcus radiodurans
1.8.3.1
Tris-HCl
up to a concentration of 70 mM, up to 2fold activation
Deinococcus radiodurans
Cloned(Commentary)
EC Number
Commentary
Organism
1.8.3.1
expressed in Escherichia coli strains BL21-CodonPlus(DE3)-RIL and TP1000
Deinococcus radiodurans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.3.1
EDTA
20 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
imidazole
0.1 mM, complete inhibition
Deinococcus radiodurans
1.8.3.1
KCl
30 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
KNO3
3 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
NaCl
150 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
NiCl2
0.1 mM, complete inhibition
Deinococcus radiodurans
1.8.3.1
potassium phosphate
20 mM, pH 8.0, 50% inhibition
Deinococcus radiodurans
1.8.3.1
Sodium arsenate
7 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
Sodium sulfate
20 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
Tris-acetate
80 mM, pH 8.0, 50% inhibition
Deinococcus radiodurans
1.8.3.1
Tris-HCl
100 mM, pH 8.0, 50% inhibition
Deinococcus radiodurans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.3.1
0.0945
-
sulfite
-
Deinococcus radiodurans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.3.1
NaCl
up to a concentration of 100 mM, up to 2fold activation
Deinococcus radiodurans
1.8.3.1
Sodium arsenate
up to a concentration of 5 mM, up to 2fold activation
Deinococcus radiodurans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.3.1
38940
-
calculated from sequence of cDNA
Deinococcus radiodurans
1.8.3.1
40250
-
2 * 40250, gel filtration
Deinococcus radiodurans
1.8.3.1
43000
-
His-tagged enzyme, SDS-PAGE
Deinococcus radiodurans
1.8.3.1
44300
-
gel filtration
Deinococcus radiodurans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.3.1
Deinococcus radiodurans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.3.1
His-Select HF nickel affinity gel column chromatography
Deinococcus radiodurans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.3.1
sulfite + H2O + A
-
669129
Deinococcus radiodurans
SO42- + AH2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.3.1
dimer
2 * 40250, gel filtration
Deinococcus radiodurans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.3.1
55
-
-
Deinococcus radiodurans
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.8.3.1
30
60
-
Deinococcus radiodurans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.3.1
45
60
half-lives of 10 min at 60°C, 30 min at 55°C, and 3 h at 45°C, respectively
Deinococcus radiodurans
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.8.3.1
Deinococcus radiodurans
-
-
6.09
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.3.1
Tris-acetate
up to a concentration of 70 mM, up to 2fold activation
Deinococcus radiodurans
1.8.3.1
Tris-HCl
up to a concentration of 70 mM, up to 2fold activation
Deinococcus radiodurans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.3.1
expressed in Escherichia coli strains BL21-CodonPlus(DE3)-RIL and TP1000
Deinococcus radiodurans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.3.1
EDTA
20 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
imidazole
0.1 mM, complete inhibition
Deinococcus radiodurans
1.8.3.1
KCl
30 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
KNO3
3 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
NaCl
150 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
NiCl2
0.1 mM, complete inhibition
Deinococcus radiodurans
1.8.3.1
potassium phosphate
20 mM, pH 8.0, 50% inhibition
Deinococcus radiodurans
1.8.3.1
Sodium arsenate
7 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
Sodium sulfate
20 mM, 50% inhibition
Deinococcus radiodurans
1.8.3.1
Tris-acetate
80 mM, pH 8.0, 50% inhibition
Deinococcus radiodurans
1.8.3.1
Tris-HCl
100 mM, pH 8.0, 50% inhibition
Deinococcus radiodurans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.3.1
0.0945
-
sulfite
-
Deinococcus radiodurans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.3.1
NaCl
up to a concentration of 100 mM, up to 2fold activation
Deinococcus radiodurans
1.8.3.1
Sodium arsenate
up to a concentration of 5 mM, up to 2fold activation
Deinococcus radiodurans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.3.1
38940
-
calculated from sequence of cDNA
Deinococcus radiodurans
1.8.3.1
40250
-
2 * 40250, gel filtration
Deinococcus radiodurans
1.8.3.1
43000
-
His-tagged enzyme, SDS-PAGE
Deinococcus radiodurans
1.8.3.1
44300
-
gel filtration
Deinococcus radiodurans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.3.1
His-Select HF nickel affinity gel column chromatography
Deinococcus radiodurans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.3.1
sulfite + H2O + A
-
669129
Deinococcus radiodurans
SO42- + AH2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.3.1
dimer
2 * 40250, gel filtration
Deinococcus radiodurans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.3.1
55
-
-
Deinococcus radiodurans
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.8.3.1
30
60
-
Deinococcus radiodurans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.3.1
45
60
half-lives of 10 min at 60°C, 30 min at 55°C, and 3 h at 45°C, respectively
Deinococcus radiodurans
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.8.3.1
Deinococcus radiodurans
-
-
6.09