BRENDA - Reference Id = 669129

Identification and characterization of a novel bacterial sulfite oxidase with no heme binding domain from Deinococcus radiodurans

D'Errico, G.; Di Salle, A.; La Cara, F.; Rossi, M.; Cannio, R.; J. Bacteriol. 188, 694-701 (2006)

Data extracted from this reference:

Activating Compound

1.8.3.1

Tris-acetate

up to a concentration of 70 mM, up to 2fold activation

Deinococcus radiodurans

1.8.3.1

Tris-HCl

up to a concentration of 70 mM, up to 2fold activation

Deinococcus radiodurans

Cloned(Commentary)

1.8.3.1

expressed in Escherichia coli strains BL21-CodonPlus(DE3)-RIL and TP1000

Deinococcus radiodurans

Inhibitors

1.8.3.1

EDTA

20 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

imidazole

0.1 mM, complete inhibition

Deinococcus radiodurans

1.8.3.1

KCl

30 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

KNO3

3 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

NaCl

150 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

NiCl2

0.1 mM, complete inhibition

Deinococcus radiodurans

1.8.3.1

potassium phosphate

20 mM, pH 8.0, 50% inhibition

Deinococcus radiodurans

1.8.3.1

Sodium arsenate

7 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

Sodium sulfate

20 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

Tris-acetate

80 mM, pH 8.0, 50% inhibition

Deinococcus radiodurans

1.8.3.1

Tris-HCl

100 mM, pH 8.0, 50% inhibition

Deinococcus radiodurans

KM Value [mM]

1.8.3.1

0.0945

sulfite

Deinococcus radiodurans

Localization

Metals/Ions

1.8.3.1

NaCl

up to a concentration of 100 mM, up to 2fold activation

Deinococcus radiodurans

1.8.3.1

Sodium arsenate

up to a concentration of 5 mM, up to 2fold activation

Deinococcus radiodurans

Molecular Weight [Da]

1.8.3.1

38940

calculated from sequence of cDNA

Deinococcus radiodurans

1.8.3.1

40250

2 * 40250, gel filtration

Deinococcus radiodurans

1.8.3.1

43000

His-tagged enzyme, SDS-PAGE

Deinococcus radiodurans

1.8.3.1

44300

gel filtration

Deinococcus radiodurans

Organism

1.8.3.1

Deinococcus radiodurans

Purification (Commentary)

1.8.3.1

His-Select HF nickel affinity gel column chromatography

Deinococcus radiodurans

Substrates and Products (Substrate)

1.8.3.1

sulfite + H2O + A

669129

Deinococcus radiodurans

SO42- + AH2

Subunits

1.8.3.1

dimer

2 * 40250, gel filtration

Deinococcus radiodurans

Synonyms

1.8.3.1

Deinococcus radiodurans

Temperature Optimum [°C]

1.8.3.1

Deinococcus radiodurans

Temperature Range [°C]

1.8.3.1

Deinococcus radiodurans

Temperature Stability [°C]

1.8.3.1

half-lives of 10 min at 60°C, 30 min at 55°C, and 3 h at 45°C, respectively

Deinococcus radiodurans

pI Value

1.8.3.1

Deinococcus radiodurans

6.09

Activating Compound (protein specific)

1.8.3.1

Tris-acetate

up to a concentration of 70 mM, up to 2fold activation

Deinococcus radiodurans

1.8.3.1

Tris-HCl

up to a concentration of 70 mM, up to 2fold activation

Deinococcus radiodurans

Cloned(Commentary) (protein specific)

1.8.3.1

expressed in Escherichia coli strains BL21-CodonPlus(DE3)-RIL and TP1000

Deinococcus radiodurans

Inhibitors (protein specific)

1.8.3.1

EDTA

20 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

imidazole

0.1 mM, complete inhibition

Deinococcus radiodurans

1.8.3.1

KCl

30 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

KNO3

3 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

NaCl

150 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

NiCl2

0.1 mM, complete inhibition

Deinococcus radiodurans

1.8.3.1

potassium phosphate

20 mM, pH 8.0, 50% inhibition

Deinococcus radiodurans

1.8.3.1

Sodium arsenate

7 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

Sodium sulfate

20 mM, 50% inhibition

Deinococcus radiodurans

1.8.3.1

Tris-acetate

80 mM, pH 8.0, 50% inhibition

Deinococcus radiodurans

1.8.3.1

Tris-HCl

100 mM, pH 8.0, 50% inhibition

Deinococcus radiodurans

KM Value [mM] (protein specific)

1.8.3.1

0.0945

sulfite

Deinococcus radiodurans

Localization (protein specific)

Metals/Ions (protein specific)

1.8.3.1

NaCl

up to a concentration of 100 mM, up to 2fold activation

Deinococcus radiodurans

1.8.3.1

Sodium arsenate

up to a concentration of 5 mM, up to 2fold activation

Deinococcus radiodurans

Molecular Weight [Da] (protein specific)

1.8.3.1

38940

calculated from sequence of cDNA

Deinococcus radiodurans

1.8.3.1

40250

2 * 40250, gel filtration

Deinococcus radiodurans

1.8.3.1

43000

His-tagged enzyme, SDS-PAGE

Deinococcus radiodurans

1.8.3.1

44300

gel filtration

Deinococcus radiodurans

Purification (Commentary) (protein specific)

1.8.3.1

His-Select HF nickel affinity gel column chromatography

Deinococcus radiodurans

Substrates and Products (Substrate) (protein specific)

1.8.3.1

sulfite + H2O + A

669129

Deinococcus radiodurans

SO42- + AH2

Subunits (protein specific)

1.8.3.1

dimer

2 * 40250, gel filtration

Deinococcus radiodurans

Temperature Optimum [°C] (protein specific)

1.8.3.1

Deinococcus radiodurans

Temperature Range [°C] (protein specific)

1.8.3.1

Deinococcus radiodurans

Temperature Stability [°C] (protein specific)

1.8.3.1

half-lives of 10 min at 60°C, 30 min at 55°C, and 3 h at 45°C, respectively

Deinococcus radiodurans

pI Value (protein specific)

1.8.3.1

Deinococcus radiodurans

6.09