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Literature summary extracted from

  • D'Errico, G.; Di Salle, A.; La Cara, F.; Rossi, M.; Cannio, R.
    Identification and characterization of a novel bacterial sulfite oxidase with no heme binding domain from Deinococcus radiodurans (2006), J. Bacteriol., 188, 694-701.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.3.1 Tris-acetate up to a concentration of 70 mM, up to 2fold activation Deinococcus radiodurans
1.8.3.1 Tris-HCl up to a concentration of 70 mM, up to 2fold activation Deinococcus radiodurans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.1 expressed in Escherichia coli strains BL21-CodonPlus(DE3)-RIL and TP1000 Deinococcus radiodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.1 EDTA 20 mM, 50% inhibition Deinococcus radiodurans
1.8.3.1 imidazole 0.1 mM, complete inhibition Deinococcus radiodurans
1.8.3.1 KCl 30 mM, 50% inhibition Deinococcus radiodurans
1.8.3.1 KNO3 3 mM, 50% inhibition Deinococcus radiodurans
1.8.3.1 NaCl 150 mM, 50% inhibition Deinococcus radiodurans
1.8.3.1 NiCl2 0.1 mM, complete inhibition Deinococcus radiodurans
1.8.3.1 potassium phosphate 20 mM, pH 8.0, 50% inhibition Deinococcus radiodurans
1.8.3.1 Sodium arsenate 7 mM, 50% inhibition Deinococcus radiodurans
1.8.3.1 Sodium sulfate 20 mM, 50% inhibition Deinococcus radiodurans
1.8.3.1 Tris-acetate 80 mM, pH 8.0, 50% inhibition Deinococcus radiodurans
1.8.3.1 Tris-HCl 100 mM, pH 8.0, 50% inhibition Deinococcus radiodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.1 0.0945
-
 sulfite
-
 Deinococcus radiodurans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.1 NaCl up to a concentration of 100 mM, up to 2fold activation Deinococcus radiodurans
1.8.3.1 Sodium arsenate up to a concentration of 5 mM, up to 2fold activation Deinococcus radiodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.1 38940
-
 calculated from sequence of cDNA Deinococcus radiodurans
1.8.3.1 40250
-
 2 * 40250, gel filtration Deinococcus radiodurans
1.8.3.1 43000
-
 His-tagged enzyme, SDS-PAGE Deinococcus radiodurans
1.8.3.1 44300
-
 gel filtration Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.1 Deinococcus radiodurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.1 His-Select HF nickel affinity gel column chromatography Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.1 sulfite + H2O + A
-
 Deinococcus radiodurans SO42- + AH2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.3.1 dimer 2 * 40250, gel filtration Deinococcus radiodurans

Synonyms

EC Number Synonyms Comment Organism
1.8.3.1 SO
-
 Deinococcus radiodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.3.1 55
-
-
 Deinococcus radiodurans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.8.3.1 30 60
-
 Deinococcus radiodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.3.1 45 60 half-lives of 10 min at 60°C, 30 min at 55°C, and 3 h at 45°C, respectively Deinococcus radiodurans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.3.1 Deinococcus radiodurans
-
-
 6.09