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Literature summary extracted from

  • Colabroy, K.L.; Begley, T.P.
    Tryptophan catabolism: Identification and characterization of a new degradative pathway (2005), J. Bacteriol., 187, 7866-7869.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.99.5 degradation new tryptophan catabolic pathway in Burholderia cepacia J2315, formation of the intermediate 4-oxalocrotonate differentiates this pathway from the proposed mammalian pathway which converts 2-aminomuconate to 2-ketoadipate and, ultimately, glutaryl-coenzyme A Burkholderia cepacia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.32 expression as His-tag fusion protein in Escherichia coli Tuner (DE3) Burkholderia cepacia
1.2.1.32 into the plasmid pDESTF1, which encodes an N-terminal six-His tag and is under control of the T7lac promoter, is used to transform Escherichia coli Tuner DE3 Burkholderia cepacia
3.5.99.5 cloned into plasmid pDESTF1 and overexpressed in Escherichia coli Tuner (DE3) Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.32 57000
-
 SDS-PAGE Burkholderia cepacia
1.2.1.32 57000
-
 SDS-PAGE, recombinant protein Burkholderia cepacia
3.5.99.5 18000
-
 overexpressed soluble protein Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O Burkholderia cepacia
-
 2-aminomuconate + NADH + H+
-
 ?
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O Burkholderia cepacia J2315
-
 2-aminomuconate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.32 Burkholderia cepacia
-
-
-
1.2.1.32 Burkholderia cepacia J2315
-
-
-
3.5.99.5 Burkholderia cepacia
-
-
-
3.5.99.5 Burkholderia cepacia J2315
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.32 by nickelnitrilotriacetic acid affinity chromatography Burkholderia cepacia
1.2.1.32 Ni-affinity chromatography Burkholderia cepacia
3.5.99.5 by nickel-nitrilotriacetic acid affinity chromatography Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O
-
 Burkholderia cepacia 2-aminomuconate + NADH + H+
-
 ?
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O
-
 Burkholderia cepacia J2315 2-aminomuconate + NADH + H+
-
 ?
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O
-
 Burkholderia cepacia 2-aminomuconate + NADH + 2 H+
-
 ?
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O
-
 Burkholderia cepacia J2315 2-aminomuconate + NADH + 2 H+
-
 ?
3.5.99.5 2-aminomuconate + H2O
-
 Burkholderia cepacia 4-oxalocrotonate + NH3
-
 ?
3.5.99.5 2-aminomuconate + H2O
-
 Burkholderia cepacia J2315 4-oxalocrotonate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.32 2-aminomuconate semialdehyde dehydrogenase
-
 Burkholderia cepacia
1.2.1.32 AMDH
-
 Burkholderia cepacia
1.2.1.32 aminomuconate-semialdehyde dehydrogenase
-
 Burkholderia cepacia
3.5.99.5 2-aminomuconate deaminase
-
 Burkholderia cepacia
3.5.99.5 AMD
-
 Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.32 NAD+
-
 Burkholderia cepacia