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Literature summary extracted from

  • Gaspar, A.H.; Marraffini, L.A.; Glass, E.M.; Debord, K.L.; Ton-That, H.; Schneewind, O.
    Bacillus anthracis sortase A (SrtA) anchors LPXTG motif-containing surface proteins to the cell wall envelope (2005), J. Bacteriol., 187, 4646-4655.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.22.70 medicine Bacillus anthracis SrtA anchors surface proteins bearing LPXTG motif sorting signals to the cell wall envelope of vegetative bacilli, srtA gene of Bacillus anthracis is not required for the development of acute anthrax disease in A/J mice Bacillus anthracis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 cloned into the plasmid vector pQE30 Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.70 additional information srtA mutants AHG263 (srtA gene deleted by allelic replacement with ermC marker) and AHG188 Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.70 (2-(trimethylammonium)ethyl)methanethiosulfonate inhibition at 5 mM, inhibition is relieved by supplementing the reaction with 10 mM dithiothreitol Bacillus anthracis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.70 23000
-
 SDS-PAGE, immune-reactive species Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Bacillus anthracis
-
 parent strain Sterne 34F2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70 by Ni-NTA affinity chromatography Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 2-aminobenzoyl-LPATG-diaminopropionic acid + H2O efficient cleavage Bacillus anthracis 2-aminobenzoyl-LPAT + G-diaminopropionic acid
-
 ?
3.4.22.70 2-aminobenzoyl-LPNTA-diaminopropionic acid + H2O small amount of cleavage Bacillus anthracis 2-aminobenzoyl-LPNT + A-diaminopropionic acid
-
 ?
3.4.22.70 Abz-LPETG-Dap(Dnp) + H2O cleavage between the threonine and the glycine residues, efficient cleavage Bacillus anthracis Abz-LPET + G-Dap(Dnp)
-
 ?
3.4.22.70 additional information no cleavage of 2-aminobenzoyl-NPKTG-diaminopropionic acid and 2-aminobenzoyl-LGATG-diaminopropionic acid Bacillus anthracis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.70 SrtA
-
 Bacillus anthracis