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Literature summary extracted from

  • Xu, W.; Jones, C.R.; Dunn, C.A.; Bessman, M.J.
    Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily (2004), J. Bacteriol., 186, 8380-8384.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.5 Bacillus subtilis gene ytkD cloned and expressed in Escherichia coli Bacillus subtilis
3.6.1.15 gene ytkD, expression in Escherichia coli strain HMS174, complementation of the mutT mutator phenotype in Escherichia coli strain SB3 lacking a functional mutT gene Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.16
-
 dGTP pH 8.5, 37°C Bacillus subtilis
3.6.1.5 0.43
-
 8-oxo-dGTP pH 8.5, 37°C Bacillus subtilis
3.6.1.5 0.89
-
 dATP pH 8.5, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Mg2+
-
 Bacillus subtilis
3.6.1.5 Mn2+ 15% residual activity compared with Mg2+ Bacillus subtilis
3.6.1.15 Mg2+
-
 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Bacillus subtilis
-
-
-
3.6.1.15 Bacillus subtilis
-
 gene ytkD
-
3.6.1.15 Bacillus subtilis 168
-
 gene ytkD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.15 recombinant enzyme from Escherichia coli strain HMS174 by streptomycin sulfate treatment and gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 8-oxo-dGTP + 2 H2O
-
 Bacillus subtilis 8-oxo-dGMP + 2 phosphate
-
 ?
3.6.1.5 adenosine (5')-tetraphospho-(5')-adenosine + H2O
-
 Bacillus subtilis ?
-
 ?
3.6.1.5 ADP-ribose + H2O
-
 Bacillus subtilis ?
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Bacillus subtilis AMP + 2 phosphate
-
 ?
3.6.1.5 CDP-choline + H2O
-
 Bacillus subtilis ?
-
 ?
3.6.1.5 CDP-choline + H2O
-
 Bacillus subtilis 168 ?
-
 ?
3.6.1.5 CTP + 2 H2O
-
 Bacillus subtilis CMP + 2 phosphate
-
 ?
3.6.1.5 dATP + H2O
-
 Bacillus subtilis dAMP + phosphate + H+
-
 ?
3.6.1.5 dCTP + H2O
-
 Bacillus subtilis dCMP + phosphate + H+
-
 ?
3.6.1.5 dGTP + H2O
-
 Bacillus subtilis dGMP + phosphate + H+
-
 ?
3.6.1.5 dTTP + H2O
-
 Bacillus subtilis dTMP + phosphate + H+
-
 ?
3.6.1.5 dUTP + H2O
-
 Bacillus subtilis dUMP + phosphate + H+
-
 ?
3.6.1.5 FAD + H2O
-
 Bacillus subtilis ?
-
 ?
3.6.1.5 FAD + H2O
-
 Bacillus subtilis 168 ?
-
 ?
3.6.1.5 GDP-mannose + H2O
-
 Bacillus subtilis ?
-
 ?
3.6.1.5 GDP-mannose + H2O
-
 Bacillus subtilis 168 ?
-
 ?
3.6.1.5 GTP + 2 H2O
-
 Bacillus subtilis GMP + 2 phosphate
-
 ?
3.6.1.5 NADH + H2O
-
 Bacillus subtilis ?
-
 ?
3.6.1.5 UDP-glucose + H2O
-
 Bacillus subtilis ?
-
 ?
3.6.1.5 UTP + 2 H2O
-
 Bacillus subtilis UMP + 2 phosphate
-
 ?
3.6.1.5 UTP + 2 H2O
-
 Bacillus subtilis 168 UMP + 2 phosphate
-
 ?
3.6.1.15 dNTP + H2O
-
 Bacillus subtilis dNDP + phosphate
-
 ?
3.6.1.15 dNTP + H2O
-
 Bacillus subtilis 168 dNDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.15 More the enzyme is a member of the Nudix hydrolase superfamily Bacillus subtilis
3.6.1.15 nucleoside triphosphatase
-
 Bacillus subtilis
3.6.1.15 YtkD
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.15 37
-
 assay at Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.5 0.74
-
 dGTP pH 8.5, 37°C Bacillus subtilis
3.6.1.5 0.96
-
 8-oxo-dGTP pH 8.5, 37°C Bacillus subtilis
3.6.1.5 1.5
-
 dATP pH 8.5, 37°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.15 8.5
-
 assay at Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.5 1.6
-
 dATP pH 8.5, 37°C Bacillus subtilis
3.6.1.5 2.4
-
 8-oxo-dGTP pH 8.5, 37°C Bacillus subtilis
3.6.1.5 4.6
-
 dGTP pH 8.5, 37°C Bacillus subtilis