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Literature summary extracted from

  • Romero, P.; Lopez, R.; Garcia, E.
    Characterization of LytA-like N-acetylmuramoyl-L-alanine amidases from two new Streptococcus mitis bacteriophages provides insights into the properties of the major pneumococcal autolysin (2004), J. Bacteriol., 186, 8229-8239.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.28 gene lytAB6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of the phage enzyme in Escherichia coli strain DH10B Streptococcus mitis
3.5.1.28 gene lytAHER, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of the phage enzyme in Escherichia coli strain DH10B Streptococcus mitis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.28 choline IC50: 0.4 mM; IC50: 2 mM Streptococcus mitis
3.5.1.28 deoxycholate at 1%; at 1% Streptococcus mitis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.28 membrane the enzyme posseses transmembrane segments Streptococcus mitis 16020
-
3.5.1.28 membrane the enzyme possesses transmembrane segments Streptococcus mitis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.28 36782
-
 x * 36782, sequence calculation Streptococcus mitis
3.5.1.28 36919
-
 x * 36919, sequence calculation Streptococcus mitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.28 additional information Streptococcus mitis the enzyme performs cell wall lysis ?
-
 ?
3.5.1.28 additional information Streptococcus mitis HER1055 the enzyme performs cell wall lysis ?
-
 ?
3.5.1.28 additional information Streptococcus mitis B6 the enzyme performs cell wall lysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.28 Streptococcus mitis Q706F5 Streptococcus mitis bacteriophage PHIB6
-
3.5.1.28 Streptococcus mitis Q706F9 Streptococcus mitis bacteriophage PHIHER; isolated from Streptococcus mitis strain HER1055
-
3.5.1.28 Streptococcus mitis B6 Q706F5 Streptococcus mitis bacteriophage PHIB6
-
3.5.1.28 Streptococcus mitis HER1055 Q706F9 Streptococcus mitis bacteriophage PHIHER; isolated from Streptococcus mitis strain HER1055
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.28 recombinant phage enzyme from Escherichia coli Streptococcus mitis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.28 0.0000044
-
-
 Streptococcus mitis
3.5.1.28 0.0000046
-
-
 Streptococcus mitis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.28 additional information the enzyme performs cell wall lysis Streptococcus mitis ?
-
 ?
3.5.1.28 additional information the enzyme performs cell wall lysis Streptococcus mitis HER1055 ?
-
 ?
3.5.1.28 additional information the enzyme performs cell wall lysis Streptococcus mitis B6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.28 ? x * 36782, sequence calculation Streptococcus mitis
3.5.1.28 ? x * 36919, sequence calculation Streptococcus mitis

Synonyms

EC Number Synonyms Comment Organism
3.5.1.28 LytA-like N-acetylmuramoyl-L-alanine amidase
-
 Streptococcus mitis
3.5.1.28 LytAB6
-
 Streptococcus mitis
3.5.1.28 LytAHER
-
 Streptococcus mitis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.28 5.5
-
-
 Streptococcus mitis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.28 0.4
-
 IC50: 0.4 mM Streptococcus mitis choline
3.5.1.28 2
-
 IC50: 2 mM Streptococcus mitis choline