Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chohnan, S.; Shiraki, K.; Yokota, K.; Ohshima, M.; Kuroiwa, N.; Ahmed, K.; Masaki, T.; Sakiyama, F.
    A second lysine-specific serine protease from Lysobacter sp. strain IB-9374 (2004), J. Bacteriol., 186, 5093-5100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.50
-
 Lysobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.50 diisopropyl fluorophosphate
-
 Lysobacter sp.
3.4.21.50 Guanidine-HCl 0.1 M, 20 min, 72% loss of activity Lysobacter sp.
3.4.21.50 N-tosyl-L-Lys chloromethylketone
-
 Lysobacter sp.
3.4.21.50 sodium dodecylsulfate 0.1%, 20 min, 70% loss of activity Lysobacter sp.
3.4.21.50 Urea 4 M, 20 min, 80% loss of activity Lysobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.50 32000
-
 x * 32000, SDS-PAGE Lysobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.50 Lysobacter sp. Q8GQZ0 isoform LepB
-
3.4.21.50 Lysobacter sp. IB-9374 Q8GQZ0 isoform LepB
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.50 proteolytic modification sequence contains a prepetide of 20 amino acids, a propeptide of 184 amino acids and a mature enzyme of 274 amino acids with a C-terminal extension peptide of 200 amino acids Lysobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.50 4.3
-
 pH 9.2, 30°C Lysobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.50 benzoyl-L-Lys-4-nitroanilide + H2O
-
 Lysobacter sp. benzoyl-L-Lys + 4-nitroaniline
-
 ?
3.4.21.50 benzoyl-L-Lys-4-nitroanilide + H2O
-
 Lysobacter sp. IB-9374 benzoyl-L-Lys + 4-nitroaniline
-
 ?
3.4.21.50 Glucagon + H2O specific hydrolysis of lysyl peptide bond Lysobacter sp. ?
-
 ?
3.4.21.50 Glucagon + H2O specific hydrolysis of lysyl peptide bond Lysobacter sp. IB-9374 ?
-
 ?
3.4.21.50 L-Lys-4-nitroanilide + H2O slow reaction Lysobacter sp. L-Lys + 4-nitroaniline
-
 ?
3.4.21.50 L-Lys-4-nitroanilide + H2O slow reaction Lysobacter sp. IB-9374 L-Lys + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.50 ? x * 32000, SDS-PAGE Lysobacter sp.
3.4.21.50 More isozyme of LepA, can fold into a three-dimensional structure quite similar to LepA Lysobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.50 40
-
-
 Lysobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.50 30
-
 moderately stable up to Lysobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.50 7 10 more than 50% of maximum activity in this range Lysobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.50 4 11 4°C, stable for at least 24 h Lysobacter sp.