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Literature summary extracted from

  • Rosner, B.M.; Schink, B.
    Purification and characterization of acetylene hydratase of Pelobacter acetylenicus, a tungsten iron-sulfur protein (1995), J. Bacteriol., 177, 5767-5772.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.112 Dithionite a strong reductant is required for activity Syntrophotalea acetylenica
4.2.1.112 additional information no activation by tungstate at 5 mM Syntrophotalea acetylenica
4.2.1.112 Ti(III)citrate a strong reductant is required for activity Syntrophotalea acetylenica

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.112 CO 90% inhibition at 0.8 mM Syntrophotalea acetylenica
4.2.1.112 HgCl2 reduces enzyme activity by 40% at 0.01 mM, 80% at 0.1 mM, and 98% at 0.2 mM Syntrophotalea acetylenica
4.2.1.112 KCN 20% inhibition at 1 mM, 40% inhibition at 5-10 mM Syntrophotalea acetylenica
4.2.1.112 molybdate
-
 Syntrophotalea acetylenica
4.2.1.112 additional information no inhibition by citrate, ascorbate, tiron, ferrocene, and EDTA, no inhibition by ethylene at 8 mM Syntrophotalea acetylenica
4.2.1.112 nitric oxide complete inhibition at 3 mM Syntrophotalea acetylenica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.112 additional information
-
 additional information the enzyme activity depends on the redox status, overview Syntrophotalea acetylenica
4.2.1.112 0.014
-
 Acetylene pH 7.0, 30°C Syntrophotalea acetylenica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.112 Iron-sulfur cluster dependent on, the enzyme is a tungsten/iron-sulfur protein, 4.8 mol of iron per mol of enzyme and 3.9 mol od acid-labile sulfur per mol of enzyme Syntrophotalea acetylenica
4.2.1.112 additional information no molybdenum detectable Syntrophotalea acetylenica
4.2.1.112 Tungsten dependent on, the enzyme is a tungsten/iron-sulfur protein, 0.4 mol of tungsten per mol of enzyme Syntrophotalea acetylenica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.112 60000
-
 gel filtration Syntrophotalea acetylenica
4.2.1.112 73000
-
 1 * 73000, SDS-PAGE Syntrophotalea acetylenica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.112 acetylene + H2O Syntrophotalea acetylenica
-
 acetaldehyde
-
 ?
4.2.1.112 acetylene + H2O Syntrophotalea acetylenica WoAcy1 / DSM 3246
-
 acetaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.112 Syntrophotalea acetylenica
-
-
-
4.2.1.112 Syntrophotalea acetylenica WoAcy1 / DSM 3246
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.112 under air at room temperature, native enzyme 240-fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and a second anion exchange chromatography step to homogeneity Syntrophotalea acetylenica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.112 culture condition:acetylene-grown cell
-
 Syntrophotalea acetylenica
-
4.2.1.112 culture condition:molybdate-grown cell
-
 Syntrophotalea acetylenica
-
4.2.1.112 culture condition:tungstate-grown cell
-
 Syntrophotalea acetylenica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.112 69.2
-
 purified enzyme Syntrophotalea acetylenica

Storage Stability

EC Number Storage Stability Organism
4.2.1.112 enzyme activity is stable even after prolonged storage of the cell extract or of the purified protein under air Syntrophotalea acetylenica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.112 acetylene + H2O
-
 Syntrophotalea acetylenica acetaldehyde
-
 ?
4.2.1.112 acetylene + H2O
-
 Syntrophotalea acetylenica WoAcy1 / DSM 3246 acetaldehyde
-
 ?
4.2.1.112 additional information no activity with ethylene, methylene blue, or anthraquinone disulfonate, the purified enzyme has no coenzyme A-acetylating aldehyde dehydrogenase activity Syntrophotalea acetylenica ?
-
 ?
4.2.1.112 additional information no activity with ethylene, methylene blue, or anthraquinone disulfonate, the purified enzyme has no coenzyme A-acetylating aldehyde dehydrogenase activity Syntrophotalea acetylenica WoAcy1 / DSM 3246 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.112 monomer 1 * 73000, SDS-PAGE Syntrophotalea acetylenica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.112 50
-
-
 Syntrophotalea acetylenica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.112 22 80
-
 Syntrophotalea acetylenica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.112 6 6.5
-
 Syntrophotalea acetylenica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.112 4 9
-
 Syntrophotalea acetylenica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.112 Syntrophotalea acetylenica chromatofocusing
-
 4.3