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Literature summary extracted from
- Inhibitory activity of chlorogenic acid on enzymes involved in the fatty acid synthesis in animals and bacteria (2006), IUBMB Life, 58, 39-46.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.100 | gene fabG, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.100 | chlorogenic acid | IC50: 0.881-0.948 mM | Escherichia coli |  |
| 1.1.1.100 | NADP+ | competitive product inhibition | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.100 | Escherichia coli | - |
gene fabG | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.100 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.100 | acetoacetyl-CoA + NADPH | - |
Escherichia coli | 3-hydroxybutyryl-CoA + NADP+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.100 | beta-ketoacyl-ACP reductase | - |
Escherichia coli |
| 1.1.1.100 | FabG | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.100 | 37 | - |
assay at | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.100 | 7 | - |
assay at | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.100 | NADPH | - |
Escherichia coli | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.100 | additional information | - |
additional information | inhibition kinetic analysis | Escherichia coli |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.100 | 0.881 | 0.948 | IC50: 0.881-0.948 mM | Escherichia coli | chlorogenic acid | |
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