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Literature summary extracted from

  • Aranda, A.; Maugeri, D.; Uttaro, A.D.; Opperdoes, F.; Cazzulo, J.J.; Nowicki, C.
    The malate dehydrogenase isoforms from Trypanosoma brucei: subcellular localization and differential expression in bloodstream and procyclic forms (2006), Int. J. Parasitol., 36, 295-307.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.37 mitochondrial and glycosomal malate dehydrogenase isoenzyme, functional expression in Escherichia coli Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.015
-
 oxaloacetate glycosomal enzyme Trypanosoma brucei
1.1.1.37 0.036
-
 NADH glycosomal enzyme Trypanosoma brucei
1.1.1.37 0.054
-
 NADH pH 7.5, 37°C, glycosomal enzyme Trypanosoma brucei
1.1.1.37 0.069
-
 oxaloacetate glycosomal enzyme Trypanosoma brucei
1.1.1.37 0.085
-
 NADH pH 7.5, 37°C, glycosomal enzyme Trypanosoma brucei
1.1.1.37 0.32
-
 NADH glacosomal enzyme Trypanosoma brucei
1.1.1.37 0.56
-
 (S)-malate pH 7.5, 37°C, mitochondrial enzyme Trypanosoma brucei
1.1.1.37 2.7
-
 p-hydroxyphenylpyruvate
-
 Trypanosoma brucei
1.1.1.37 2.72
-
 (S)-malate pH 7.5, 37°C, glycosomal enzyme Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytosol cytosolic isoenzyme is the only isoform of malate dehydrogenase expressed in the bloodstream form Trypanosoma brucei 5829
-
1.1.1.37 glycosome
-
 Trypanosoma brucei 20015
-
1.1.1.37 mitochondrion
-
 Trypanosoma brucei 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ Trypanosoma brucei mitochondrial enzyme, Krebs cycle oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH Trypanosoma brucei cytosolic enzyme and glycosomal enzyme (S)-malate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Trypanosoma brucei O15769
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 bloodstream form the cytosolic isoenzyme is the only isoform of malate dehydrogenase expressed in the bloodstream form Trypanosoma brucei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ mitochondrial enzyme, Krebs cycle Trypanosoma brucei oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH cytosolic enzyme and glycosomal enzyme Trypanosoma brucei (S)-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH + H+
-
 Trypanosoma brucei (S)-malate + NAD+
-
 r
1.1.1.37 p-hydroxyphenylpyruvate + NADH + H+
-
 Trypanosoma brucei 2-hydroxy-3-(4-hydroxyphenyl)propanoate
-
 ?
1.1.1.37 phenylpyruvate + NADH + H+
-
 Trypanosoma brucei 2-hydroxy-3-phenylpropanoate + NAD+
-
 ?