BRENDA - Enzyme Database

Purification, properties and alternate substrate specificities of arginase from two different sources: Vigna catjang cotyledon and buffalo liver

Dabir, S.; Dabir, P.; Somvanshi, B.; Int. J. Biol. Sci. 1, 114-122 (2005)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.1
2
-
L-arginine
pH 9.2, 37C
Bos taurus
3.5.3.1
42
-
L-arginine
pH 10.0, 37C
Vigna catjang
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.1
Ca2+
52% of the activity with Mn2+
Bos taurus
3.5.3.1
Ca2+
35% of the activity with Mn2+
Vigna catjang
3.5.3.1
Co2+
45% of the activity with Mn2+
Bos taurus
3.5.3.1
Co2+
66% of the activity with Mn2+
Vigna catjang
3.5.3.1
Fe2+
52% of the activity with Mn2+
Vigna catjang
3.5.3.1
Mg2+
93% of the activity with Mn2+
Bos taurus
3.5.3.1
Mg2+
61% of the activity with Mn2+
Vigna catjang
3.5.3.1
Mn2+
maximal activity at 2.0 mM
Bos taurus
3.5.3.1
Mn2+
maximal activity at 0.6 mM
Vigna catjang
3.5.3.1
additional information
no activity with Fe2+, Zn2+
Bos taurus
3.5.3.1
Ni2+
51% of the activity with Mn2+
Bos taurus
3.5.3.1
Ni2+
55% of the activity with Mn2+
Vigna catjang
3.5.3.1
Zn2+
38% of the activity with Mn2+
Vigna catjang
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.1
43000
-
x * 43000, SDS-PAGE
Bos taurus
3.5.3.1
52000
-
4 * 52000, SDS-PAGE
Vigna catjang
3.5.3.1
120000
-
gel filtration
Bos taurus
3.5.3.1
210000
-
gel filtration
Vigna catjang
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.1
Bos taurus
-
-
-
3.5.3.1
Vigna catjang
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.1
-
Vigna catjang
3.5.3.1
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.1
cotyledon
-
Vigna catjang
-
3.5.3.1
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.3.1
2045
-
pH 10.0, 37C
Vigna catjang
3.5.3.1
91300
-
pH 9.2, 37C
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.1
agmatine + H2O
16% of the activity with L-arginine
668975
Vigna catjang
1,4-diaminobutane + urea
-
-
-
?
3.5.3.1
canavanine + H2O
12.7% of the activity with L-arginine
668975
Vigna catjang
urea + NH2OCH2CH2CH(NH2)COOH
-
-
-
?
3.5.3.1
canavanine + H2O
9.6% of the activity with L-arginine
668975
Bos taurus
urea + NH2OCH2CH2CH(NH2)COOH
-
-
-
?
3.5.3.1
L-arginine + H2O
-
668975
Bos taurus
L-ornithine + urea
-
-
-
?
3.5.3.1
L-arginine + H2O
-
668975
Vigna catjang
L-ornithine + urea
-
-
-
?
3.5.3.1
additional information
no substrate: agmatine, D-arginine, L-homoarginine, L-argininic acid, gamma-guanidinobutyric acid, beta-guanidinopropionic acid, streptomycin
668975
Bos taurus
?
-
-
-
-
3.5.3.1
additional information
no substrate: D-arginine, L-homoarginine, L-argininic acid, gamma-guanidinobutyric acid, beta-guanidinopropionic acid, streptomycin
668975
Vigna catjang
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.1
?
x * 43000, SDS-PAGE
Bos taurus
3.5.3.1
tetramer
4 * 52000, SDS-PAGE
Vigna catjang
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.3.1
35
-
-
Vigna catjang
3.5.3.1
42
-
-
Bos taurus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.3.1
60
-
stable
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.1
10
-
-
Bos taurus
3.5.3.1
10
-
-
Vigna catjang
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.1
2
-
L-arginine
pH 9.2, 37C
Bos taurus
3.5.3.1
42
-
L-arginine
pH 10.0, 37C
Vigna catjang
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.1
Ca2+
52% of the activity with Mn2+
Bos taurus
3.5.3.1
Ca2+
35% of the activity with Mn2+
Vigna catjang
3.5.3.1
Co2+
45% of the activity with Mn2+
Bos taurus
3.5.3.1
Co2+
66% of the activity with Mn2+
Vigna catjang
3.5.3.1
Fe2+
52% of the activity with Mn2+
Vigna catjang
3.5.3.1
Mg2+
93% of the activity with Mn2+
Bos taurus
3.5.3.1
Mg2+
61% of the activity with Mn2+
Vigna catjang
3.5.3.1
Mn2+
maximal activity at 2.0 mM
Bos taurus
3.5.3.1
Mn2+
maximal activity at 0.6 mM
Vigna catjang
3.5.3.1
additional information
no activity with Fe2+, Zn2+
Bos taurus
3.5.3.1
Ni2+
51% of the activity with Mn2+
Bos taurus
3.5.3.1
Ni2+
55% of the activity with Mn2+
Vigna catjang
3.5.3.1
Zn2+
38% of the activity with Mn2+
Vigna catjang
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.1
43000
-
x * 43000, SDS-PAGE
Bos taurus
3.5.3.1
52000
-
4 * 52000, SDS-PAGE
Vigna catjang
3.5.3.1
120000
-
gel filtration
Bos taurus
3.5.3.1
210000
-
gel filtration
Vigna catjang
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.1
-
Vigna catjang
3.5.3.1
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.1
cotyledon
-
Vigna catjang
-
3.5.3.1
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.3.1
2045
-
pH 10.0, 37C
Vigna catjang
3.5.3.1
91300
-
pH 9.2, 37C
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.1
agmatine + H2O
16% of the activity with L-arginine
668975
Vigna catjang
1,4-diaminobutane + urea
-
-
-
?
3.5.3.1
canavanine + H2O
12.7% of the activity with L-arginine
668975
Vigna catjang
urea + NH2OCH2CH2CH(NH2)COOH
-
-
-
?
3.5.3.1
canavanine + H2O
9.6% of the activity with L-arginine
668975
Bos taurus
urea + NH2OCH2CH2CH(NH2)COOH
-
-
-
?
3.5.3.1
L-arginine + H2O
-
668975
Bos taurus
L-ornithine + urea
-
-
-
?
3.5.3.1
L-arginine + H2O
-
668975
Vigna catjang
L-ornithine + urea
-
-
-
?
3.5.3.1
additional information
no substrate: agmatine, D-arginine, L-homoarginine, L-argininic acid, gamma-guanidinobutyric acid, beta-guanidinopropionic acid, streptomycin
668975
Bos taurus
?
-
-
-
-
3.5.3.1
additional information
no substrate: D-arginine, L-homoarginine, L-argininic acid, gamma-guanidinobutyric acid, beta-guanidinopropionic acid, streptomycin
668975
Vigna catjang
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.1
?
x * 43000, SDS-PAGE
Bos taurus
3.5.3.1
tetramer
4 * 52000, SDS-PAGE
Vigna catjang
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.3.1
35
-
-
Vigna catjang
3.5.3.1
42
-
-
Bos taurus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.3.1
60
-
stable
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.1
10
-
-
Bos taurus
3.5.3.1
10
-
-
Vigna catjang