Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rangarajan, M.; Hashim, A.; Aduse-Opoku, J.; Paramonov, N.; Hounsell, E.F.; Curtis, M.A.
    Expression of Arg-gingipain RgpB is required for correct glycosylation and stability of monomeric Arg-gingipain RgpA from Porphyromonas gingivalis W50 (2005), Infect. Immun., 73, 4864-4878.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.37 additional information mutant RgpA-deficient isogenic strain W501 and RgpB-deficient isogenic strain D7 show 50% of wild-type strain W50 enzyme activity Porphyromonas gingivalis

General Stability

EC Number General Stability Organism
3.4.22.37 expression of RgpB is required for correct glycosylation and stability of monomeric RgpA from strain W50 Porphyromonas gingivalis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.37 Porphyromonas gingivalis
-
 genes rgpA and rgpB, wild-type strain W50, RgpA-deficient isogenic strain W501, and RgpB-deficient isogenic strain D7
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.37 glycoprotein monosaccharide composition of RgpA, expression of RgpB is required for correct glycosylation and stability of monomeric RgpA from strain W50 Porphyromonas gingivalis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.37 native enzymes by ammonium sulfate fractionation, ion exchange and affinity chromatography, RgpA and RgpB by different methods, overview Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.37 Nalpha-benzoyl-D,L-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis Nalpha-benzoyl-D,L-Arg + 4-nitroaniline
-
 ?
3.4.22.37 Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
-
 Porphyromonas gingivalis Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.37 dimer heterodimeric RgpA fused to adhesin, i.e. HRgpA Porphyromonas gingivalis
3.4.22.37 monomer RgpA Porphyromonas gingivalis

Synonyms

EC Number Synonyms Comment Organism
3.4.22.37 Arg-gingipain
-
 Porphyromonas gingivalis
3.4.22.37 RgpA
-
 Porphyromonas gingivalis
3.4.22.37 RgpB
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.37 30
-
 assay at Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.37 8.1
-
 assay at Porphyromonas gingivalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.37 7.8 8.3 stability of RgpA catalytic domain of wild-type and D7 mutant strain at 30°C in absence or presence of 2-mercaptoethanol or Ca2+, overview Porphyromonas gingivalis