Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yasumasu, S.; Yamagami, K.
    Choriolysin L (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 623-625.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.24.66 degradation first choriolysin H swells the inner layer of egg envelope by limited digestion, and then choriolysin L solubilizes the swollen part of it completely Oryzias latipes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.66 EDTA inhibits at 1 mM Oryzias latipes
3.4.24.66 additional information not inhibited by DFP, iodoacetamide or iodoacetic acid Oryzias latipes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.66 Cu2+ partly reactivates EDTA-inactivated apoenzyme Oryzias latipes
3.4.24.66 Zn2+ reactivates EDTA-inactivated apoenzyme Oryzias latipes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.66 25500
-
-
 Oryzias latipes

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.66 Oryzias latipes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.66 partly purified by gel filtration and cation-exchange column Oryzias latipes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.66 embryo
-
 Oryzias latipes
-
3.4.24.66 hatching gland colocalized with choriolysin H in the secretory granules Oryzias latipes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.66 casein + H2O
-
 Oryzias latipes ?
-
 ?
3.4.24.66 chorion + H2O
-
 Oryzias latipes ?
-
 ?
3.4.24.66 Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O
-
 Oryzias latipes ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.66 monomer
-
 Oryzias latipes

Synonyms

EC Number Synonyms Comment Organism
3.4.24.66 chorionase
-
 Oryzias latipes
3.4.24.66 LCE
-
 Oryzias latipes
3.4.24.66 Low choriolytic enzyme
-
 Oryzias latipes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.66 8.6
-
 for caseinolytic activity Oryzias latipes