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Literature summary extracted from

  • Gache, C.; Lepage, T.; Croce, J.; Lhomond, G.
    Envelysin (2004), Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 575-578.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.24.12 degradation one of the earliest zygotic genes activated, about 12-20 h after fertilization, the hatching enzyme is secreted, digesting the fertilization envelope and freeing the swimming blastula Echinoidea
3.4.24.12 molecular biology Ets4 is one of the transcription factors that control HE expression Strongylocentrotus purpuratus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.12
-
 Paracentrotus lividus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.12 Ac-Pro-Arg-Cys-Gly-Val-Pro-Asp-NH2
-
 Echinoidea
3.4.24.12 EDTA completely inhibited by 20 mM, inhibition is not reversible by Ca2+ Echinoidea
3.4.24.12 jaspisin
-
 Echinoidea
3.4.24.12 Li+ shifts the border of the envelysin expression domain towards the animal pole Echinoidea
3.4.24.12 TIMP-1
-
 Hemicentrotus pulcherrimus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.12 additional information envelysin is probably packaged in secretory vesicles first localized between nucleus and the apical face Echinoidea
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.12 Ca2+ required for full activity Echinoidea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.12 47700
-
-
 Echinoidea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.12 fertilization envelope + H2O Echinoidea
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.12 Echinoidea
-
-
-
3.4.24.12 Hemicentrotus pulcherrimus
-
-
-
3.4.24.12 Paracentrotus lividus
-
-
-
3.4.24.12 Strongylocentrotus purpuratus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.12 by hydrophobic chromatography Paracentrotus lividus
3.4.24.12 by hydrophobic chromatography Strongylocentrotus purpuratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.12 embryo
-
 Echinoidea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.12 fertilization envelope + H2O
-
 Echinoidea ?
-
 ?
3.4.24.12 insulin oxidized B chain of insulin cleaved at FVNQ-HLCGSHLVEA-LY-LVCGERGFF-YTPKA Hemicentrotus pulcherrimus ?
-
 ?
3.4.24.12 additional information short proteolyzed envelysin lacking the C-terminal part preferentially cleaves peptide bonds on the amino side of hydrophobic bulky residues (-Leu,-Ile,-Phe,-Tyr) Hemicentrotus pulcherrimus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.12 monomer
-
 Echinoidea

Synonyms

EC Number Synonyms Comment Organism
3.4.24.12 hatching enzyme
-
 Hemicentrotus pulcherrimus
3.4.24.12 hatching enzyme
-
 Echinoidea
3.4.24.12 hatching enzyme
-
 Paracentrotus lividus
3.4.24.12 hatching enzyme
-
 Strongylocentrotus purpuratus
3.4.24.12 HE
-
 Strongylocentrotus purpuratus