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Literature summary extracted from

  • Ichishima, E.
    Pycnoporopepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 141-143.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.23.30 purified isozymes Ia and Ib from acetone Trametes coccinea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.30 extracellular the isozymes are secreted Trametes coccinea
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.30 33000 34000 sedimentation equilibrium centrifugation analysis Trametes coccinea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.30 additional information Trametes coccinea the extracellular enzyme is involved in fungal nutrition from wood ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.30 Trametes coccinea
-
 a wood-deteriorating basidiomycete, formerly Trametes sanguinea, isozymes Ia and Ib
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.30 native isozymes Ia and Ib 30fold to homogeneity from mycel-free culture filtrate, by acetone precipitation, anion exchange chromatograph, ammonium sulfate fractionation, dialysis and crystallization to homogeneity, and further to puritiy by anion exchange and sulfopropyl chromatograpgy, and isoelectric focusing Trametes coccinea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.30 commercial preparation crude enzyme preparation Trametes coccinea
-
3.4.23.30 mycelium submerged culture Trametes coccinea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.30 additional information
-
 purified isozyme Ia and Ib Trametes coccinea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.30 angiotensin + H2O cleavage at Tyr4-Ile5, at pH 2.7 Trametes coccinea ?
-
 ?
3.4.23.30 casein + H2O
-
 Trametes coccinea ?
-
 ?
3.4.23.30 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O i.e. oxidized insulin B chain, cleavage site specificity of isozymes Ia and Ib at pH 2.7, overview Trametes coccinea FVNQHLCGSHLVEA + Leu-Val + LVCGERGF + FYTPKA
-
 ?
3.4.23.30 Hemoglobin + H2O
-
 Trametes coccinea ?
-
 ?
3.4.23.30 additional information the extracellular enzyme is involved in fungal nutrition from wood Trametes coccinea ?
-
 ?
3.4.23.30 additional information cleavage site specificity of the isoymes, no activity with isulin A chain, overview Trametes coccinea ?
-
 ?
3.4.23.30 pro-angiotensin + H2O cleavage at Tyr4-Ile5 and Phe8-Phe9, at pH 2.7 Trametes coccinea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.30 More three-dimensional structure and active site structure Trametes coccinea

Synonyms

EC Number Synonyms Comment Organism
3.4.23.30 carboxyl proteinase I formerly Trametes coccinea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.30 2.3
-
 substrate hemoglobin Trametes coccinea
3.4.23.30 2.5
-
 substrate casein Trametes coccinea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.23.30 Trametes coccinea isozyme Ib
-
 4.6
3.4.23.30 Trametes coccinea isozyme Ia
-
 4.7