BRENDA - Enzyme Database

Rhodotorulapepsin

Murao, S.; Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ) 1, 139-140 (2004)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
3.4.23.26
purified native enzyme, 3times crystallization
Rhodotorula glutinis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.23.26
4-bromo-phenacylbromide
-
Rhodotorula glutinis
3.4.23.26
diazoacetyl glycine ethyl ester
-
Rhodotorula glutinis
3.4.23.26
Diazoacetyl-DL-norleucine methyl ester
i.e. DAN
Rhodotorula glutinis
3.4.23.26
pepstatin Ac
60% inhibition at 0.031 mM, addition to cell culture enhances the cell growth and changes the optimal growth temperature
Rhodotorula glutinis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.23.26
extracellular
-
Rhodotorula glutinis
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.23.26
29000
-
gel filtration
Rhodotorula glutinis
3.4.23.26
30000
-
sedimentation equilibrium analysis
Rhodotorula glutinis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.23.26
Rhodotorula glutinis
-
isolated from soil
-
3.4.23.26
Rhodotorula glutinis K-24
-
isolated from soil
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.23.26
native enzyme from strain K-24 by ammonium sulfate fractionation, acetone precipation, gel filtration to homogeneity
Rhodotorula glutinis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.23.26
additional information
-
-
Rhodotorula glutinis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.23.26
casein + H2O
high activity
668791
Rhodotorula glutinis
?
-
-
-
?
3.4.23.26
casein + H2O
high activity
668791
Rhodotorula glutinis K-24
?
-
-
-
?
3.4.23.26
DRVYIHPFHLLVYS + H2O
tetradecapaptide renin substrate, cleavage pattern, overview
668791
Rhodotorula glutinis
DRVY + IHPFHLL + VYS
-
-
-
?
3.4.23.26
DRVYIHPFHLLVYS + H2O
tetradecapaptide renin substrate, cleavage pattern, overview
668791
Rhodotorula glutinis K-24
DRVY + IHPFHLL + VYS
-
-
-
?
3.4.23.26
additional information
substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala
668791
Rhodotorula glutinis
?
-
-
-
?
3.4.23.26
additional information
substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala
668791
Rhodotorula glutinis K-24
?
-
-
-
?
3.4.23.26
Z-Lys-Ala-Ala-Ala + H2O
-
668791
Rhodotorula glutinis
Z-Lys + Ala-Ala-Ala
-
-
-
?
3.4.23.26
Z-Lys-Ala-Ala-Ala + H2O
-
668791
Rhodotorula glutinis K-24
Z-Lys + Ala-Ala-Ala
-
-
-
?
3.4.23.26
Z-Lys-Leu-Ala-Ala + H2O
-
668791
Rhodotorula glutinis
Z-Lys + Leu-Ala-Ala
-
-
-
?
3.4.23.26
Z-Lys-Leu-Ala-Ala + H2O
-
668791
Rhodotorula glutinis K-24
Z-Lys + Leu-Ala-Ala
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.23.26
2
2.5
-
Rhodotorula glutinis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.4.23.26
Rhodotorula glutinis
isoelectric focusing
-
4.5
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.4.23.26
purified native enzyme, 3times crystallization
Rhodotorula glutinis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.23.26
4-bromo-phenacylbromide
-
Rhodotorula glutinis
3.4.23.26
diazoacetyl glycine ethyl ester
-
Rhodotorula glutinis
3.4.23.26
Diazoacetyl-DL-norleucine methyl ester
i.e. DAN
Rhodotorula glutinis
3.4.23.26
pepstatin Ac
60% inhibition at 0.031 mM, addition to cell culture enhances the cell growth and changes the optimal growth temperature
Rhodotorula glutinis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.23.26
extracellular
-
Rhodotorula glutinis
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.23.26
29000
-
gel filtration
Rhodotorula glutinis
3.4.23.26
30000
-
sedimentation equilibrium analysis
Rhodotorula glutinis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.23.26
native enzyme from strain K-24 by ammonium sulfate fractionation, acetone precipation, gel filtration to homogeneity
Rhodotorula glutinis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.23.26
additional information
-
-
Rhodotorula glutinis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.23.26
casein + H2O
high activity
668791
Rhodotorula glutinis
?
-
-
-
?
3.4.23.26
casein + H2O
high activity
668791
Rhodotorula glutinis K-24
?
-
-
-
?
3.4.23.26
DRVYIHPFHLLVYS + H2O
tetradecapaptide renin substrate, cleavage pattern, overview
668791
Rhodotorula glutinis
DRVY + IHPFHLL + VYS
-
-
-
?
3.4.23.26
DRVYIHPFHLLVYS + H2O
tetradecapaptide renin substrate, cleavage pattern, overview
668791
Rhodotorula glutinis K-24
DRVY + IHPFHLL + VYS
-
-
-
?
3.4.23.26
additional information
substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala
668791
Rhodotorula glutinis
?
-
-
-
?
3.4.23.26
additional information
substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala
668791
Rhodotorula glutinis K-24
?
-
-
-
?
3.4.23.26
Z-Lys-Ala-Ala-Ala + H2O
-
668791
Rhodotorula glutinis
Z-Lys + Ala-Ala-Ala
-
-
-
?
3.4.23.26
Z-Lys-Ala-Ala-Ala + H2O
-
668791
Rhodotorula glutinis K-24
Z-Lys + Ala-Ala-Ala
-
-
-
?
3.4.23.26
Z-Lys-Leu-Ala-Ala + H2O
-
668791
Rhodotorula glutinis
Z-Lys + Leu-Ala-Ala
-
-
-
?
3.4.23.26
Z-Lys-Leu-Ala-Ala + H2O
-
668791
Rhodotorula glutinis K-24
Z-Lys + Leu-Ala-Ala
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.23.26
2
2.5
-
Rhodotorula glutinis
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.4.23.26
Rhodotorula glutinis
isoelectric focusing
-
4.5