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Literature summary extracted from

  • Murao, S.
    Rhodotorulapepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 139-140.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.23.26 purified native enzyme, 3times crystallization Rhodotorula glutinis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.26 4-bromo-phenacylbromide
-
 Rhodotorula glutinis
3.4.23.26 diazoacetyl glycine ethyl ester
-
 Rhodotorula glutinis
3.4.23.26 Diazoacetyl-DL-norleucine methyl ester i.e. DAN Rhodotorula glutinis
3.4.23.26 pepstatin Ac 60% inhibition at 0.031 mM, addition to cell culture enhances the cell growth and changes the optimal growth temperature Rhodotorula glutinis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.26 extracellular
-
 Rhodotorula glutinis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.26 29000
-
 gel filtration Rhodotorula glutinis
3.4.23.26 30000
-
 sedimentation equilibrium analysis Rhodotorula glutinis

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.26 Rhodotorula glutinis
-
 isolated from soil
-
3.4.23.26 Rhodotorula glutinis K-24
-
 isolated from soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.26 native enzyme from strain K-24 by ammonium sulfate fractionation, acetone precipation, gel filtration to homogeneity Rhodotorula glutinis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.26 additional information
-
-
 Rhodotorula glutinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.26 casein + H2O high activity Rhodotorula glutinis ?
-
 ?
3.4.23.26 casein + H2O high activity Rhodotorula glutinis K-24 ?
-
 ?
3.4.23.26 DRVYIHPFHLLVYS + H2O tetradecapaptide renin substrate, cleavage pattern, overview Rhodotorula glutinis DRVY + IHPFHLL + VYS
-
 ?
3.4.23.26 DRVYIHPFHLLVYS + H2O tetradecapaptide renin substrate, cleavage pattern, overview Rhodotorula glutinis K-24 DRVY + IHPFHLL + VYS
-
 ?
3.4.23.26 additional information substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala Rhodotorula glutinis ?
-
 ?
3.4.23.26 additional information substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala Rhodotorula glutinis K-24 ?
-
 ?
3.4.23.26 Z-Lys-Ala-Ala-Ala + H2O
-
 Rhodotorula glutinis Z-Lys + Ala-Ala-Ala
-
 ?
3.4.23.26 Z-Lys-Ala-Ala-Ala + H2O
-
 Rhodotorula glutinis K-24 Z-Lys + Ala-Ala-Ala
-
 ?
3.4.23.26 Z-Lys-Leu-Ala-Ala + H2O
-
 Rhodotorula glutinis Z-Lys + Leu-Ala-Ala
-
 ?
3.4.23.26 Z-Lys-Leu-Ala-Ala + H2O
-
 Rhodotorula glutinis K-24 Z-Lys + Leu-Ala-Ala
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.26 2 2.5
-
 Rhodotorula glutinis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.23.26 Rhodotorula glutinis isoelectric focusing
-
 4.5