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Literature summary extracted from

  • Cooper, J.B.
    Endothiapepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 104-107.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.23.22 nutrition the enzyme can be used as a fungal rennet in cheese production Cryphonectria parasitica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.22 DNA and amino acid sequence determination and analysis Cryphonectria parasitica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.23.22 using ammonium sulfate as precipitant at pH 4.6, X-ray diffraction structure determination and analysis at 2.1 A resolution, multiple isomorphous replacement Cryphonectria parasitica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.22 L363564 statine-containing renin inhibitor, hydrogen bonding interaction witht the enzyme, e.g. via Asp32 and Asp215, overview Cryphonectria parasitica
3.4.23.22 pepstatin A strong inhibition, the inhibitor motif -CH(OH)-CH2- mimicks the tetrahedral intermdiate of the catalytic reaction Cryphonectria parasitica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.22 33800
-
 x * 33800 Cryphonectria parasitica

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.22 Cryphonectria parasitica
-
 i.e. Cryphonectria parasitica, the chestnut blight fungus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.22 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O i.e. oxidized insulin B chain, cleavage site specificity Cryphonectria parasitica FVN + Gln + HLCGSHL + VEALY + LVCGERGF + FYTPKA
-
 ?
3.4.23.22 kappa-casein + H2O reaction contributes to milk-clotting activity, cleavage site specificity for Ser104-Phe105 Cryphonectria parasitica ?
-
 ?
3.4.23.22 additional information the enzyme prefers protein substrates with hydrophobic amino acid residues at P1 and P1' positions Cryphonectria parasitica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.22 ? x * 33800 Cryphonectria parasitica
3.4.23.22 More structure analysis, overview Cryphonectria parasitica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.22 additional information
-
 acidic pH optimum Cryphonectria parasitica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.23.22 Cryphonectria parasitica about
-
 5.5