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Literature summary extracted from
- Plant legumain, asparaginyl endopeptidase (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 1310-1313.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.34 | DTT | - |
Phaseolus vulgaris |  |
| 3.4.22.34 | DTT | - |
Glycine max | |
| 3.4.22.34 | DTT | - |
Arabidopsis thaliana | |
| 3.4.22.34 | DTT | - |
Ricinus communis | |
| 3.4.22.34 | DTT | - |
Canavalia ensiformis | |
| 3.4.22.34 | DTT | - |
Vigna aconitifolia | |
| 3.4.22.34 | DTT | - |
Vigna mungo | |
| 3.4.22.34 | EDTA | - |
Phaseolus vulgaris | |
| 3.4.22.34 | EDTA | - |
Glycine max | |
| 3.4.22.34 | EDTA | - |
Arabidopsis thaliana | |
| 3.4.22.34 | EDTA | - |
Ricinus communis | |
| 3.4.22.34 | EDTA | - |
Canavalia ensiformis | |
| 3.4.22.34 | EDTA | - |
Vigna aconitifolia | |
| 3.4.22.34 | EDTA | - |
Vigna mungo | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.22.34 | DNA and amino acid sequence determination | Ricinus communis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.34 | 4-chloromercuribenzoate | - |
Canavalia ensiformis | |
| 3.4.22.34 | additional information | no inhibition by E64 | Canavalia ensiformis | |
| 3.4.22.34 | N-ethylmaleimide | - |
Canavalia ensiformis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.22.34 | soluble | - |
Phaseolus vulgaris | - |
- |
| 3.4.22.34 | soluble | - |
Glycine max | - |
- |
| 3.4.22.34 | soluble | - |
Arabidopsis thaliana | - |
- |
| 3.4.22.34 | soluble | - |
Ricinus communis | - |
- |
| 3.4.22.34 | soluble | - |
Canavalia ensiformis | - |
- |
| 3.4.22.34 | soluble | - |
Vigna aconitifolia | - |
- |
| 3.4.22.34 | soluble | - |
Vigna mungo | - |
- |
| 3.4.22.34 | vacuole | - |
Phaseolus vulgaris | 5773 | - |
| 3.4.22.34 | vacuole | - |
Glycine max | 5773 | - |
| 3.4.22.34 | vacuole | - |
Ricinus communis | 5773 | - |
| 3.4.22.34 | vacuole | - |
Canavalia ensiformis | 5773 | - |
| 3.4.22.34 | vacuole | - |
Vigna aconitifolia | 5773 | - |
| 3.4.22.34 | vacuole | - |
Vigna mungo | 5773 | - |
| 3.4.22.34 | vacuole | the alpha and gamma vacuolar processing enzyme occurs only in vegetative organs, while the beta VPE occurs in seeds | Arabidopsis thaliana | 5773 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | 23400 | - |
1 * 23400 | Phaseolus vulgaris |
| 3.4.22.34 | 33000 | - |
1 * 33000 | Vigna aconitifolia |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.34 | additional information | Ricinus communis | the enzyme is involved in degradation of seed storage proteins | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.34 | Arabidopsis thaliana | - |
- |
- |
| 3.4.22.34 | Canavalia ensiformis | - |
jackbean | - |
| 3.4.22.34 | Glycine max | - |
- |
- |
| 3.4.22.34 | Phaseolus vulgaris | - |
- |
- |
| 3.4.22.34 | Ricinus communis | - |
- |
- |
| 3.4.22.34 | Vigna aconitifolia | - |
moth bean | - |
| 3.4.22.34 | Vigna mungo | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.34 | glycoprotein | probably N-glycosylated | Vigna aconitifolia |
| 3.4.22.34 | proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Phaseolus vulgaris |
| 3.4.22.34 | proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Arabidopsis thaliana |
| 3.4.22.34 | proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Ricinus communis |
| 3.4.22.34 | proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Canavalia ensiformis |
| 3.4.22.34 | proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Vigna aconitifolia |
| 3.4.22.34 | proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Vigna mungo |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.34 | native enzyme from dry seeds, to homogeneity | Glycine max |
| 3.4.22.34 | native enzyme from dry seeds, to homogeneity | Ricinus communis |
| 3.4.22.34 | native enzyme from dry seeds, to homogeneity | Canavalia ensiformis |
| 3.4.22.34 | native enzyme from germinated seeds, to homogeneity | Phaseolus vulgaris |
| 3.4.22.34 | native enzyme from germinated seeds, to homogeneity | Vigna aconitifolia |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.34 | endosperm | late stage of seed maturation | Ricinus communis | - |
| 3.4.22.34 | additional information | the alpha and gamma vacuolar processing enzyme occurs only in vegetative organs | Arabidopsis thaliana | - |
| 3.4.22.34 | seed | - |
Vigna aconitifolia | - |
| 3.4.22.34 | seed | beta VPE | Arabidopsis thaliana | - |
| 3.4.22.34 | seed | in endosperm of late stage of seed maturation | Ricinus communis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.34 | Boc-Asn-4-nitrophenyl ester + H2O | - |
Phaseolus vulgaris | Boc-Asn + 4-nitrophenol | - |
? | |
| 3.4.22.34 | Dnp-Pro-Glu-Ala-Asn-NH2 + H2O | - |
Phaseolus vulgaris | Dnp-Pro-Glu-Ala-Asn + NH3 | - |
? | |
| 3.4.22.34 | ETRNGVEE + H2O | - |
Phaseolus vulgaris | ETRN + GVEE | - |
? | |
| 3.4.22.34 | ETRNGVEE + H2O | - |
Glycine max | ETRN + GVEE | - |
? | |
| 3.4.22.34 | additional information | the enzyme is involved in degradation of seed storage proteins | Ricinus communis | ? | - |
? | |
| 3.4.22.34 | additional information | the enzyme hydrolyzes peptide bonds with Asn in P1 | Phaseolus vulgaris | ? | - |
? | |
| 3.4.22.34 | additional information | the enzyme hydrolyzes peptide bonds with Asn in P1 and all 20 amino acids in P1' including S-alkylated Cys, with a few exceptions | Canavalia ensiformis | ? | - |
? | |
| 3.4.22.34 | SESENGLEET + H2O | - |
Phaseolus vulgaris | SESEN + GLEET | - |
? | |
| 3.4.22.34 | SESENGLEET + H2O | - |
Glycine max | SESEN + GLEET | - |
? | |
| 3.4.22.34 | Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Phaseolus vulgaris | Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.34 | monomer | 1 * 33000 | Vigna aconitifolia |
| 3.4.22.34 | monomer | 1 * 23400 | Phaseolus vulgaris |
| 3.4.22.34 | monomer | 1 * 33000-33800 | Glycine max |
| 3.4.22.34 | More | primary structure determination | Ricinus communis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.22.34 | Asparaginyl endopeptidase | - |
Phaseolus vulgaris |
| 3.4.22.34 | Asparaginyl endopeptidase | - |
Glycine max |
| 3.4.22.34 | Asparaginyl endopeptidase | - |
Arabidopsis thaliana |
| 3.4.22.34 | Asparaginyl endopeptidase | - |
Ricinus communis |
| 3.4.22.34 | Asparaginyl endopeptidase | - |
Canavalia ensiformis |
| 3.4.22.34 | Asparaginyl endopeptidase | - |
Vigna aconitifolia |
| 3.4.22.34 | Asparaginyl endopeptidase | - |
Vigna mungo |
| 3.4.22.34 | vacuolar processing enzyme | - |
Phaseolus vulgaris |
| 3.4.22.34 | vacuolar processing enzyme | - |
Glycine max |
| 3.4.22.34 | vacuolar processing enzyme | - |
Arabidopsis thaliana |
| 3.4.22.34 | vacuolar processing enzyme | - |
Ricinus communis |
| 3.4.22.34 | vacuolar processing enzyme | - |
Canavalia ensiformis |
| 3.4.22.34 | vacuolar processing enzyme | - |
Vigna aconitifolia |
| 3.4.22.34 | vacuolar processing enzyme | - |
Vigna mungo |
| 3.4.22.34 | VPE | - |
Phaseolus vulgaris |
| 3.4.22.34 | VPE | - |
Glycine max |
| 3.4.22.34 | VPE | - |
Arabidopsis thaliana |
| 3.4.22.34 | VPE | - |
Ricinus communis |
| 3.4.22.34 | VPE | - |
Canavalia ensiformis |
| 3.4.22.34 | VPE | - |
Vigna aconitifolia |
| 3.4.22.34 | VPE | - |
Vigna mungo |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | 5 | 5.5 | - |
Phaseolus vulgaris |
| 3.4.22.34 | 5 | 5.5 | - |
Glycine max |
| 3.4.22.34 | 5 | 5.5 | - |
Arabidopsis thaliana |
| 3.4.22.34 | 5 | 5.5 | - |
Ricinus communis |
| 3.4.22.34 | 5 | 5.5 | - |
Canavalia ensiformis |
| 3.4.22.34 | 5 | 5.5 | - |
Vigna aconitifolia |
| 3.4.22.34 | 5 | 5.5 | - |
Vigna mungo |
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