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Literature summary extracted from
- Plasminogen activator of Yersinia pestis (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 1, 217-219.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.48 | 0.000145 | - |
plasminogen | - |
Yersinia pestis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.23.48 | membrane | outer membrane protein | Yersinia pestis | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.48 | 31000 | - |
plasminogen activator Pla exhibits three molecular forms: alpha-Pla with an MW of 35000 Da, beta-Pla of 33000 Da and gamma-Pla with 31000 Da. the formation of beta-Pla from alpha-Pla results from autoprocessing at the residue Lys262. gamma-Pla most likely represents full-size mature Pla that has folded differently from alpha-Pla | Yersinia pestis |
| 3.4.23.48 | 33000 | - |
plasminogen activator Pla exhibits three molecular forms: alpha-Pla with an MW of 35000 Da, beta-Pla of 33000 Da and gamma-Pla with 31000 Da. the formation of beta-Pla from alpha-Pla results from autoprocessing at the residue Lys262. gamma-Pla most likely represents full-size mature Pla that has folded differently from alpha-Pla | Yersinia pestis |
| 3.4.23.48 | 35000 | - |
plasminogen activator Pla exhibits three molecular forms: alpha-Pla with an MW of 35000 Da, beta-Pla of 33000 Da and gamma-Pla with 31000 Da. the formation of beta-Pla from alpha-Pla results from autoprocessing at the residue Lys262. gamma-Pla most likely represents full-size mature Pla that has folded differently from alpha-Pla | Yersinia pestis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.48 | additional information | Yersinia pestis | plasminogen activator Pla is essential for the spread of Yersinia pestis from the subcutaneous infection site into circulation, and the proteolytic activation of plasminogen is involved in virulence function. In addition to proteolysis plasminogen activator Pla promotes bacterial adhesion to mammalian extracellular matrices and epithelial cells as well as bacterial invasion into eukayotic cells. Laminin functions as an adhesion target for plasminogen activator Pla, which also expresses a lower adhesion affinity for heparan sulfate proteoglycan. the adhesion targets are not directly degraded by plasminogen activator Pla, but Pla-mediated generation of plasmin leads to their degradation | ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.48 | Yersinia pestis | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.23.48 | proteolytic modification | plasminogen activator Pla is autoprocessed at the C-terminus. This does not enhance plasminogen activation | Yersinia pestis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.48 | complement protein C3 + H2O | - |
Yersinia pestis | ? | - |
? | |
| 3.4.23.48 | additional information | plasminogen activator Pla is essential for the spread of Yersinia pestis from the subcutaneous infection site into circulation, and the proteolytic activation of plasminogen is involved in virulence function. In addition to proteolysis plasminogen activator Pla promotes bacterial adhesion to mammalian extracellular matrices and epithelial cells as well as bacterial invasion into eukayotic cells. Laminin functions as an adhesion target for plasminogen activator Pla, which also expresses a lower adhesion affinity for heparan sulfate proteoglycan. the adhesion targets are not directly degraded by plasminogen activator Pla, but Pla-mediated generation of plasmin leads to their degradation | Yersinia pestis | ? | - |
? | |
| 3.4.23.48 | additional information | no degradation of Val-Leu-Lys-p-nitroanilide. Plasminogen activator degrades outer membrane proteins of Yersinia cell surface and exhibits a weak coagulase activity. In addition to proteolysis plasminogen activator Pla promotes bacterial adhesion to mammalian extracellular matrices and epithelial cells as well as bacterial invasion into eukaryotic cells. Laminin functions as an adhesion target for plasminogen activator Pla, which also expresses a lower adhesion affinity for heparan sulfate proteoglycan. The adhesion targets are not directly degraded by plasminogen activator Pla, but Pla-mediated generation of plasmin leads to their degradation | Yersinia pestis | ? | - |
? | |
| 3.4.23.48 | plasminogen + H2O | cleavage of Arg560-/-Val561 | Yersinia pestis | plasmin + ? | - |
? | |
| 3.4.23.48 | serpin alpha2-antiplasmin + H2O | - |
Yersinia pestis | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.48 | 0.0035 | - |
plasminogen | - |
Yersinia pestis |
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