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Literature summary extracted from
- Characteristics of carboxypeptidase B from pyloric ceca of the starfish Asterina pectinifera (2005), Food Chem., 95, 264-269.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.2 | EDTA | 6 h, 1 mM, 90% inhibition | Patiria pectinifera |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.2 | Co2+ | activates 1.7fold at 25°C and pH 7.5 | Patiria pectinifera | |
| 3.4.17.2 | Zn2+ | 1 mol Zn2+ per mol of enzyme | Patiria pectinifera | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 34000 | - |
x * 34000, SDS-PAGE | Patiria pectinifera |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.2 | Patiria pectinifera | - |
starfish | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.2 | native enzyme 418fold from pyloric ceca to near homogeneity by gel filtration, anion exchange chromatography, and another step of gel filtration | Patiria pectinifera |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.17.2 | pyloric cecum | - |
Patiria pectinifera | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | additional information | - |
- |
Patiria pectinifera |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.2 | benzoyl-Gly-L-Arg + H2O | - |
Patiria pectinifera | benzoyl-Gly + L-Arg | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.17.2 | ? | x * 34000, SDS-PAGE | Patiria pectinifera |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.17.2 | CPB | - |
Patiria pectinifera |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 55 | - |
- |
Patiria pectinifera |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 30 | 70 | inactivation above 50°C | Patiria pectinifera |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 30 | 45 | 15 min, purified enzyme, stable | Patiria pectinifera |
| 3.4.17.2 | 50 | - |
15 min, the purified enzyme is unstable above | Patiria pectinifera |
| 3.4.17.2 | 60 | - |
15 min, loss of 70% of purified enzyme activity | Patiria pectinifera |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 7.5 | - |
- |
Patiria pectinifera |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 4 | 11 | high loss of activity below pH 6.0 and above pH 10.0 | Patiria pectinifera |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 4 | - |
30 min, inactivation | Patiria pectinifera |
| 3.4.17.2 | 5 | - |
the purified enzyme is unstable below | Patiria pectinifera |
| 3.4.17.2 | 6 | 10 | purified enzyme, stable | Patiria pectinifera |
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Release 2023.1 (January 2023)
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