Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cai, G.; Zhu, S.; Wang, X.; Jiang, W.
    Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from Achromobacter xylosoxidans (2005), FEMS Microbiol. Lett., 249, 15-21.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.4 gene ana, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the gene might be part of a transposon structure, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strains DH5alpha and JM109 Achromobacter xylosoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 0.315
-
 5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid pH 7.5, 37°C Achromobacter xylosoxidans
3.5.1.4 0.958
-
 glutaryl-nitro-5-amino benzoic acid pH 7.5, 37°C Achromobacter xylosoxidans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Achromobacter xylosoxidans Q5YFS2 gene ana
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to 99% homogeneity Achromobacter xylosoxidans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.4 additional information optimal growth temperature for enzyme production is 26°C Achromobacter xylosoxidans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 3.5
-
 purified recombinant enzyme, substrate glutaryl-nitro-5-amino benzoic acid Achromobacter xylosoxidans
3.5.1.4 4.67
-
 purified recombinant enzyme, substrate adipoyl-nitro-5-amino benzoic acid Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 5-[(4-nitrophenyl)amino]-5-oxopentanoic acid + H2O
-
 Achromobacter xylosoxidans glutarate + 4-nitroaniline
-
 ?
3.5.1.4 5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid + H2O
-
 Achromobacter xylosoxidans 2-nitrobenzoate + adipate
-
 ?
3.5.1.4 adipyl-4-nitro-aniline + H2O
-
 Achromobacter xylosoxidans ?
-
 ?
3.5.1.4 glutaryl-2-naphthylamine + H2O
-
 Achromobacter xylosoxidans ?
-
 ?
3.5.1.4 glutaryl-aniline + H2O
-
 Achromobacter xylosoxidans ?
-
 ?
3.5.1.4 glutaryl-nitro-5-amino benzoic acid + H2O
-
 Achromobacter xylosoxidans ?
-
 ?
3.5.1.4 additional information substrate specificity towards cephalosporin analogues, overview, no activity with cephalosporin C Achromobacter xylosoxidans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.4 acylase
-
 Achromobacter xylosoxidans
3.5.1.4 Ana
-
 Achromobacter xylosoxidans
3.5.1.4 More the enzyme belongs to the amidase signature superfamily Achromobacter xylosoxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 44
-
-
 Achromobacter xylosoxidans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.4 37 50 drastic drop in activity above 50°C, maximal activity at 44°C, assay at 37°C Achromobacter xylosoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 8
-
-
 Achromobacter xylosoxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 6 8 drastic drop in activity below pH 6.0, maximal activity at pH 8.0 Achromobacter xylosoxidans