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Literature summary extracted from

  • Herga, S.; Berrin, J.G.; Perrier, J.; Puigserver, A.; Giardina, T.
    Identification of the zinc binding ligands and the catalytic residue in human aspartoacylase, an enzyme involved in Canavan disease (2006), FEBS Lett., 580, 5899-5904.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.15 expression of wild-type and mutant enzymes as GST-fusion proteins in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.15 E178D site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme Homo sapiens
3.5.1.15 E178Q site-directed mutagenesis, inactive mutant Homo sapiens
3.5.1.15 E24A site-directed mutagenesis, inactive mutant Homo sapiens
3.5.1.15 H116A site-directed mutagenesis, inactive mutant Homo sapiens
3.5.1.15 H21A site-directed mutagenesis, inactive mutant Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.15 0.2
-
 N-acetylaspartic acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
3.5.1.15 0.22
-
 N-acetylaspartic acid pH 7.4, 37°C, recombinant mutant E178D Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.15 Zn2+ metallopeptidase, the enzyme contains the conserved H21XXE24(91aa)H116 motif, ligand binding by His21, Glu24, and His116, molecular modeling Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.15 36000
-
 x * 36000, recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.15 N-acetylaspartic acid + H2O Homo sapiens enzyme deficiency causes the Canavan disease, an autosomal-recessive neurodegenerative disorder L-asparatate + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.15 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.15 recombinant wild-type and mutant GST-fusion enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.15 N-acyl-L-aspartate + H2O = a carboxylate + L-aspartate reaction mechanism involving Glu178, molecular modeling Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.15 brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.15 300
-
 purified recombinant mutant E178D Homo sapiens
3.5.1.15 1557
-
 purified recombinant wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.15 N-acetylaspartic acid + H2O
-
 Homo sapiens L-asparatate + acetate
-
 ?
3.5.1.15 N-acetylaspartic acid + H2O enzyme deficiency causes the Canavan disease, an autosomal-recessive neurodegenerative disorder Homo sapiens L-asparatate + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.15 ? x * 36000, recombinant enzyme, SDS-PAGE Homo sapiens
3.5.1.15 More the enzyme belongs to the carboxypeptidase metalloprotein family with the conserved H21XXE24(91aa)H116 motif Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.1.15 ASPA
-
 Homo sapiens
3.5.1.15 More the enzyme belongs to the carboxypeptidase metalloprotein family Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.15 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.15 0.52
-
 N-acetylaspartic acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens
3.5.1.15 1.48
-
 N-acetylaspartic acid pH 7.4, 37°C, recombinant mutant E178D Homo sapiens
3.5.1.15 14.22
-
 N-acetylaspartic acid pH 7.4, 37°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.15 7.4
-
 assay at Homo sapiens