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Literature summary extracted from

  • Rajapakse, S.; Ogiwara, K.; Takano, N.; Moriyama, A.; Takahashi, T.
    Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins (2005), FEBS Lett., 579, 6879-6884.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.118 antipain 0.2 mM, 100% inhibition Homo sapiens
3.4.21.118 Aprotinin 0.01 mg/ml, 98% inhibition Homo sapiens
3.4.21.118 benzamidine 0.2 mM, 18% inhibition Homo sapiens
3.4.21.118 diisopropyl fluorophosphate 2 mM, 100% inhibition Homo sapiens
3.4.21.118 leupeptin 0.2 mM, 95% inhibition Homo sapiens
3.4.21.118 additional information not inhibitory: Nalpha-p-tosyl-L-phenylalanine chloromethyl ketone, pepstatin, o-phenynthroline, E-64 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.118 0.07
-
 L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 0.07
-
 benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 0.07
-
 t-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 0.07
-
 t-butyloxycarbonyl-L-Phe-L-Ser-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 0.1
-
 t-butyloxycarbonyl-L-Leu-L-Lys-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 0.1
-
 t-butyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.118 31500
-
 x * 31500, SDS-PAGE of active recombinant enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.118 additional information Homo sapiens enzyme may be impicated in extracellular matrix protein degradation in the area surrounding enzyme producing cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.118 Homo sapiens O60259 active recombinant enzyme
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.4.21.118 -20°C, active recombinant enzyme, stable for at least two months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.118 benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Val-L-Val-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.118 casein + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.118 collagen type IV + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.118 Fibrinogen + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.118 Fibronectin + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.118 Gelatin + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.118 high-molecular weight kininogen + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.118 L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O best substrate Homo sapiens L-Pro-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.118 additional information enzyme may be impicated in extracellular matrix protein degradation in the area surrounding enzyme producing cells Homo sapiens ?
-
 ?
3.4.21.118 additional information trypsin-like specificity with strong preference for Arg over Lys in P1-position. No substrate: plasminogen Homo sapiens ?
-
 ?
3.4.21.118 single-chain tissue-type plasminogen activator + H2O specific cleavage of Arg275-Ile276 bond Homo sapiens two-chain tissue-type plasminogen activator
-
 ?
3.4.21.118 t-butyloxycarbonyl-L-Leu-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens t-butyloxycarbonyl-L-Leu-L-Lys-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.118 t-butyloxycarbonyl-L-Phe-L-Ser-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens t-butyloxycarbonyl-L-Phe-L-Ser-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.118 t-butyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens t-butyloxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.118 t-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens t-butyloxycarbonyl-L-Val-L-Pro-L-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.118 ? x * 31500, SDS-PAGE of active recombinant enzyme Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.118 40
-
 t-butyloxycarbonyl-L-Phe-L-Ser-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 48
-
 t-butyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 65
-
 t-butyloxycarbonyl-L-Leu-L-Lys-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 98
-
 t-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 140
-
 benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens
3.4.21.118 180
-
 L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin 37°C, pH 8.0 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.118 8.5
-
-
 Homo sapiens