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Literature summary extracted from

  • Rakitina, D.V.; Kantidze, O.L.; Leshchiner, A.D.; Solovyev, A.G.; Novikov, V.K.; Morozov, S.Y.; Kalinina, N.O.
    Coat proteins of two filamentous plant viruses display NTPase activity in vitro (2005), FEBS Lett., 579, 4955-4960.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.15 functional overexpression of the His6-tagged coat protein in Escherichia coli potato virus A
3.6.1.15 functional overexpression of the His6-tagged coat protein in Escherichia coli potato virus X

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.15 additional information virus C-terminal regions deletion diminishes the ATPase activity potato virus X

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.15 EDTA
-
 potato virus A
3.6.1.15 EDTA
-
 potato virus X
3.6.1.15 rNTP ribotrinucleotides inhibit the activity with gammaATP potato virus A
3.6.1.15 rNTP ribotrinucleotides inhibit the activity with gammaATP potato virus X

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.15 Ca2+ activates slightly, much less efficient than Mg2+ potato virus A
3.6.1.15 Ca2+ activates, less efficient than Mg2+ potato virus X
3.6.1.15 Cu2+ activates slightly, much less efficient than Mg2+ potato virus A
3.6.1.15 Cu2+ activates slightly, much less efficient than Mg2+ potato virus X
3.6.1.15 Mg2+ best metal ion for enzyme activation potato virus A
3.6.1.15 Mg2+ best metal ion for enzyme activation potato virus X
3.6.1.15 Mn2+ activates, less efficient than Mg2+ potato virus A
3.6.1.15 Mn2+ activates, less efficient than Mg2+ potato virus X
3.6.1.15 additional information the enzyme is dependent on divalent cations potato virus A
3.6.1.15 additional information the enzyme is dependent on divalent cations potato virus X
3.6.1.15 Zn2+ activates slightly, much less efficient than Mg2+ potato virus A
3.6.1.15 Zn2+ activates slightly, much less efficient than Mg2+ potato virus X

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.15 ATP + H2O potato virus A
-
 ADP + phosphate
-
 ?
3.6.1.15 ATP + H2O potato virus X
-
 ADP + phosphate
-
 ?
3.6.1.15 additional information potato virus A the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response ?
-
 ?
3.6.1.15 additional information potato virus X the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.15 no activity in tobacco mosaic virus
-
-
-
3.6.1.15 potato virus A
-
 PVA, a poteyvirus
-
3.6.1.15 potato virus X
-
 PVX, a potexvirus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.15 recombinant His6-tagged coat protein from Escherichia coli by nickel affinity chromatogryphy potato virus A
3.6.1.15 recombinant His6-tagged coat protein from Escherichia coli by nickel affinity chromatogryphy potato virus X

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.15 additional information
-
-
 potato virus A
3.6.1.15 additional information
-
-
 potato virus X

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.15 ATP + H2O
-
 potato virus A ADP + phosphate
-
 ?
3.6.1.15 ATP + H2O
-
 potato virus X ADP + phosphate
-
 ?
3.6.1.15 additional information the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response potato virus A ?
-
 ?
3.6.1.15 additional information the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response potato virus X ?
-
 ?
3.6.1.15 additional information the enzyme hydrolyzes ribonucleotides and deoxyribonucleotides potato virus X ?
-
 ?
3.6.1.15 UTP + H2O recombinant enzyme potato virus A UDP + phosphate
-
 ?
3.6.1.15 UTP + H2O recombinant enzyme potato virus X UDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.15 NTPase
-
 potato virus A
3.6.1.15 NTPase
-
 potato virus X
3.6.1.15 PVA coat protein
-
 potato virus A
3.6.1.15 PVA CP
-
 potato virus A
3.6.1.15 PVx coat protein
-
 potato virus X
3.6.1.15 PVX CP
-
 potato virus X