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Literature summary extracted from

  • Sasaki, K.; Ohara, K.; Yazaki, K.
    Gene expression and characterization of isoprene synthase from Populus alba (2005), FEBS Lett., 579, 2514-2518.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.27 additional information the enzyme is induced by heat stress and continuous light irradiation Populus alba

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.27 gene ispS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, expression as GFP-fusion protein in onion peels and tobacco leaves, transformations by particle bombardement Populus alba

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.27 additional information enzyme expression is highly reduced in the dark Populus alba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.27 plastid the enzyme contains a plastidial targeting sequence Populus alba 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.27 Co2+ activates, 23% of the activity with Mg2+ Populus alba
4.2.3.27 Fe2+ 2% of the activity with Mg2+ Populus alba
4.2.3.27 Mg2+ activates at 20 mM, best divalent cation Populus alba
4.2.3.27 Mn2+ activates, 34% of the activity with Mg2+ Populus alba
4.2.3.27 additional information the enzyme requires divalent cations, no activity with Cu2+ and Ca2+ Populus alba
4.2.3.27 Ni2+ 2% of the activity with Mg2+ Populus alba
4.2.3.27 Zn2+ 3% of the activity with Mg2+ Populus alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Populus alba
-
 isoprene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Populus alba Q50L36 gene ispS; gene ispS
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.3.27 proteolytic modification the enzyme contains a plastidial targeting sequence Populus alba

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.27 recombinant enzyme from Escherichia coli Populus alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.27 cell culture from leaves Populus alba
-
4.2.3.27 leaf
-
 Populus alba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.27 0.00039
-
 leaf cell culture Populus alba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
 Populus alba isoprene + diphosphate
-
 ?
4.2.3.27 dimethylallyl diphosphate the enzyme is absolutely specific for dimethylally diphosphate Populus alba isoprene + diphosphate product identification ?
4.2.3.27 additional information substrate specifcity, no enzymatic activity with isoprenyl diphosphate or geranyl diphosphate as substrates, overview Populus alba ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.27 More the enzyme contains the aspartate-rich motif DDxxD Populus alba

Synonyms

EC Number Synonyms Comment Organism
4.2.3.27 ISPS
-
 Populus alba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.27 40
-
 recombinant enzyme Populus alba

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.3.27 25 50 about half-maximal activity at 25°C and 50°C, temperature profile Populus alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.27 8
-
 recombinant enzyme Populus alba

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.27 6.5 9.5 activity range Populus alba