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Literature summary extracted from

  • Morty, R.E.; Shih, A.Y.; Fulop, V.; Andrews, N.W.
    Identification of the reactive cysteine residues in oligopeptidase B from Trypanosoma brucei (2005), FEBS Lett., 579, 2191-2196.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.83 C256S 750fold reduction in inhibition rate constant for iodoacetic acid and more than 100fold reduction in inhibition rate constant for NEM compared to wild-type enzyme Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 iodoacetamide
-
 Trypanosoma brucei
3.4.21.83 NEM Cys256 is the reactive cysteine residue that mediates OpdB inhibition. Cys256 adducts occlude the P1 substrate-binding site, preventing substrate binding Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.83 80000
-
 x * 80000, SDS-PAGE under reducing and non-reducing conditions Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Trypanosoma brucei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.83 ? x * 80000, SDS-PAGE under reducing and non-reducing conditions Trypanosoma brucei