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Literature summary extracted from

  • Bingham, R.J.; Dive, V.; Phillips, S.E.; Shirras, A.D.; Isaac, R.E.
    Structural diversity of angiotensin-converting enzyme (2006), FEBS J., 273, 362-373.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.1 benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Ala
-
 Drosophila sp. (in: flies)
3.4.15.1 benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Trp
-
 Drosophila sp. (in: flies)
3.4.15.1 NaCl at pH 7 increasing concentrations of NaCl result in a faster rate of conversion which reaches a plateau at 150-200 mM NaCl. The activation by NaCl at pH 7 is the result of a 330% increase in kcat/Km which is solely attributable to a lowering of the Km. At pH 8, maximal activity is achieved in absence of NaCl, weak inhibitory effect on hydrolysis of angiotensin I as salt concentrations increase from 0 to 200 mM Drosophila sp. (in: flies)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.15.1 0.073
-
 Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 0.27
-
 bradykinin 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 0.3
-
 Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 0.37
-
 MKRSRGPSPRR 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 0.58
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Tyr-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 0.82
-
 angiotensin I 35°C, pH 7.0, 100 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 1.04
-
 angiotensin I 35°C, pH 8.0, 100 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 1.23
-
 angiotensin I 35°C, pH 8.0, 0 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 2.7
-
 angiotensin I 35°C, pH 7.0, 0 mM NaCl Drosophila sp. (in: flies)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.15.1 Na+ at pH 7 increasing concentrations of NaCl result in a faster rate of conversion which reaches a plateau at 150-200 mM NaCl. The activation by NaCl at pH 7 is the result of a 330% increase in kcat/Km which is solely attributable to a lowering of the Km. At pH 8, maximal activity is achieved in absence of NaCl, weak inhibitory effect on hydrolysis of angiotensin I as salt concentrations increase from 0 to 200 mM Drosophila sp. (in: flies)

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.1 Drosophila sp. (in: flies)
-
 recombinantly expressed in Pichia pastoris
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.15.1 recombinant enzyme Drosophila sp. (in: flies)

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.1 angiotensin I + H2O
-
 Drosophila sp. (in: flies) angiotensin II + His-Leu
-
 ?
3.4.15.1 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Tyr-Arg + H2O
-
 Drosophila sp. (in: flies) ?
-
 ?
3.4.15.1 Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg + H2O
-
 Drosophila sp. (in: flies) ?
-
 ?
3.4.15.1 bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg Drosophila sp. (in: flies) Arg-Pro-Pro-Gly-Phe + Ser-Pro + Phe-Arg
-
 ?
3.4.15.1 Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
-
 Drosophila sp. (in: flies) ?
-
 ?
3.4.15.1 MKRSRGPSPRR + H2O
-
 Drosophila sp. (in: flies) ?
-
 ?
3.4.15.1 [Leu5]enkephalin + H2O
-
 Drosophila sp. (in: flies) ?
-
 ?
3.4.15.1 [Leu5]enkephalinamide + H2O
-
 Drosophila sp. (in: flies) ?
-
 ?
3.4.15.1 [Met5]enkephalin + H2O
-
 Drosophila sp. (in: flies) ?
-
 ?
3.4.15.1 [Met5]enkephalinamide + H2O
-
 Drosophila sp. (in: flies) ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.15.1 ANCE
-
 Drosophila sp. (in: flies)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.15.1 0.03 0.55 angiotensin I 35°C, pH 7.0, 0 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 0.057 0.65 angiotensin I 35°C, pH 8.0, 0 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 0.057 0.65 angiotensin I 35°C, pH 8.0, 100 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 0.24
-
 Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 1.09
-
 bradykinin 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 1.1
-
 Ile-Ser-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 2.41
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Tyr-Arg 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 6.83
-
 angiotensin I 35°C, pH 7.0, 100 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 6.84
-
 angiotensin I 35°C, pH 7.0, 0 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 10.78
-
 angiotensin I 35°C, pH 8.0, 100 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 11.06
-
 angiotensin I 35°C, pH 8.0, 0 mM NaCl Drosophila sp. (in: flies)
3.4.15.1 18.8
-
 MKRSRGPSPRR 35°C, pH 8.0 Drosophila sp. (in: flies)

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.15.1 0.152
-
 benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Trp 35°C, pH 8.0 Drosophila sp. (in: flies)
3.4.15.1 0.365
-
 benzyloxycarbonyl-PhePSI[PO2-CH]Ala-Ala 35°C, pH 8.0 Drosophila sp. (in: flies)