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Literature summary extracted from

  • Kunz, S.; Oberholzer, M.; Seebeck, T.
    A FYVE-containing unusual cyclic nucleotide phosphodiesterase from Trypanosoma cruzi (2005), FEBS J., 272, 6412-6422.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.17 expression in Saccharomyces cerevisiae Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.17 3-isobutyl-1-methyl-1H-purine-2,6(3H,7H)-dione IC50: 0.068 mM Trypanosoma cruzi
3.1.4.17 dipyridamole IC50: 0.0069 mM Trypanosoma cruzi
3.1.4.17 etazolate IC50: 0.0007 mM Trypanosoma cruzi
3.1.4.17 papaverine IC50: 0.025 mM Trypanosoma cruzi
3.1.4.17 trequinsin IC50: 0.0039 mM Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.17 0.0316
-
 3',5'-cAMP
-
 Trypanosoma cruzi
3.1.4.17 0.0782
-
 3',5'-cGMP
-
 Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.17 kinetoplast
-
 Trypanosoma cruzi 20023
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.17 CaCl2 entirely dependent on the presence of divalent cations, activity with 0.2 mM CaCl2 is 15.9% of maximal activity obtained with 2 mM MgCl2 Trypanosoma cruzi
3.1.4.17 Mg2+ entirely dependent on the presence of divalent cations, maximal activity with 2 mM MgCl2 Trypanosoma cruzi
3.1.4.17 Mn2+ entirely dependent on the presence of divalent cations, activity with 2 mM MnCl2 is 53.5% of maximal activity obtained with 2 mM MgCl2 Trypanosoma cruzi
3.1.4.17 Zn2+ entirely dependent on the presence of divalent cations, activity with 0.03 mM ZnCl2 is 19.5% of maximal activity obtained with 2 mM MgCl2 Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.17 103169
-
 x * 103169, calculated from sequence Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.17 Trypanosoma cruzi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.17 3',5'-cAMP + H2O
-
 Trypanosoma cruzi 5'-AMP
-
 ?
3.1.4.17 3',5'-cGMP + H2O
-
 Trypanosoma cruzi 5'-GMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.17 ? x * 103169, calculated from sequence Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
3.1.4.17 dual-specificity PDE
-
 Trypanosoma cruzi
3.1.4.17 TcrPDEC
-
 Trypanosoma cruzi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.17 Trypanosoma cruzi calculated from sequence
-
 5.9

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.17 0.0007
-
 IC50: 0.0007 mM Trypanosoma cruzi etazolate
3.1.4.17 0.0039
-
 IC50: 0.0039 mM Trypanosoma cruzi trequinsin
3.1.4.17 0.0069
-
 IC50: 0.0069 mM Trypanosoma cruzi dipyridamole
3.1.4.17 0.025
-
 IC50: 0.025 mM Trypanosoma cruzi papaverine
3.1.4.17 0.068
-
 IC50: 0.068 mM Trypanosoma cruzi 3-isobutyl-1-methyl-1H-purine-2,6(3H,7H)-dione