Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kluskens, L.D.; van Alebeek, G.J.; Walther, J.; Voragen, A.G.; de Vos, W.M.; van der Oost, J.
    Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima (2005), FEBS J., 272, 5464-5473.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.67 expression in Escherichia coli Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 0.06
-
 (1,4-alpha-D-galacturonide)n 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.06
-
 (1,4-alpha-D-galacturonide)8 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.07
-
 (1,4-alpha-D-galacturonide)7 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.11
-
 (1,4-alpha-D-galacturonide)6 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.24
-
 (1,4-alpha-D-galacturonide)5 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.29
-
 (1,4-alpha-D-galacturonide)4 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.34
-
 (1,4-alpha-D-galacturonide)3 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.34
-
 (1,4-alpha-D-galacturonide)2 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 cytoplasm
-
 Thermotoga maritima 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 50000
-
 4 * 50000, molecular weight of the monomer deduced from sequence Thermotoga maritima
3.2.1.67 212000
-
 gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 anion-exchange chromatography Thermotoga maritima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.67 cell culture
-
 Thermotoga maritima
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 (1,4-alpha-D-galacturonide)2 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)3 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)4 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)5 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)6 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)7 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)8 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)n + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 tetramer 4 * 50000, molecular weight of the monomer deduced from sequence Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 PelB
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 80
-
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.67 182
-
 (1,4-alpha-D-galacturonide)2 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 685
-
 (1,4-alpha-D-galacturonide)3 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 821
-
 (1,4-alpha-D-galacturonide)6 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 829
-
 (1,4-alpha-D-galacturonide)4 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 843
-
 (1,4-alpha-D-galacturonide)8 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 860
-
 (1,4-alpha-D-galacturonide)7 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 934
-
 (1,4-alpha-D-galacturonide)5 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
3.2.1.67 936
-
 (1,4-alpha-D-galacturonide)n 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 6.4
-
-
 Thermotoga maritima