BRENDA - Enzyme Database

Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies. Alteration of substrate specificity by replacement of Asn149 with Asp

Lopez, V.; Alarcon, R.; Orellana, M.S.; Enriquez, P.; Uribe, E.; Martinez, J.; Carvajal, N.; FEBS J. 272, 4540-4548 (2005)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.1
H120N
53% of wild-type activity, no alteration in KM-value
Homo sapiens
3.5.3.1
H145N
95% of wild-type activity, no alteration in KM-value
Homo sapiens
3.5.3.1
N149D
almost complete loss of activity with L-arginine, gain of activity with agmatine
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.1
1.4
-
L-arginine
37°C, pH 9.0, wild-type
Homo sapiens
3.5.3.1
1.5
-
L-arginine
37°C, pH 9.0, mutant H145N
Homo sapiens
3.5.3.1
1.6
-
L-arginine
37°C, pH 9.0, mutant H120N
Homo sapiens
3.5.3.1
2.5
-
agmatine
37°C, pH 9.0, mutant N149D
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.1
Mn2+
activation. after dialysis with EDTA, wild-type and mutant H145N contain 1.1 and 1.3 Mn2+ per subunit, resp., and are hlaf-.active. Mutant H120N contains less than 0.1 Mn2+ per subunit and is inactive
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.1
Homo sapiens
-
isoform arginase II
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.1
agmatine + H2O
for wild-type, no substrate. Hydrolysis by mutant N149D
668556
Homo sapiens
1,4-diaminobutane + urea
-
-
-
?
3.5.3.1
L-arginine + H2O
-
668556
Homo sapiens
L-ornithine + urea
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.1
131
-
L-arginine
37°C, pH 9.0, mutant H120N
Homo sapiens
3.5.3.1
238
-
L-arginine
37°C, pH 9.0, mutant H145N
Homo sapiens
3.5.3.1
249
-
L-arginine
37°C, pH 9.0, wild-type
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.1
H120N
53% of wild-type activity, no alteration in KM-value
Homo sapiens
3.5.3.1
H145N
95% of wild-type activity, no alteration in KM-value
Homo sapiens
3.5.3.1
N149D
almost complete loss of activity with L-arginine, gain of activity with agmatine
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.1
1.4
-
L-arginine
37°C, pH 9.0, wild-type
Homo sapiens
3.5.3.1
1.5
-
L-arginine
37°C, pH 9.0, mutant H145N
Homo sapiens
3.5.3.1
1.6
-
L-arginine
37°C, pH 9.0, mutant H120N
Homo sapiens
3.5.3.1
2.5
-
agmatine
37°C, pH 9.0, mutant N149D
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.1
Mn2+
activation. after dialysis with EDTA, wild-type and mutant H145N contain 1.1 and 1.3 Mn2+ per subunit, resp., and are hlaf-.active. Mutant H120N contains less than 0.1 Mn2+ per subunit and is inactive
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.1
agmatine + H2O
for wild-type, no substrate. Hydrolysis by mutant N149D
668556
Homo sapiens
1,4-diaminobutane + urea
-
-
-
?
3.5.3.1
L-arginine + H2O
-
668556
Homo sapiens
L-ornithine + urea
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.1
131
-
L-arginine
37°C, pH 9.0, mutant H120N
Homo sapiens
3.5.3.1
238
-
L-arginine
37°C, pH 9.0, mutant H145N
Homo sapiens
3.5.3.1
249
-
L-arginine
37°C, pH 9.0, wild-type
Homo sapiens