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Literature summary extracted from

  • Di Salle, A.; DErrico, G.; La Cara, F.; Cannio, R.; Rossi, M.
    A novel thermostable sulfite oxidase from Thermus thermophilus: characterization of the enzyme, gene cloning and expression in Escherichia coli (2006), Extremophiles, 10, 587-598.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.3.1 EDTA
-
Thermus thermophilus
1.8.3.1 Molybdenum the addition of molybdenum to culture media at a concentration of 2.07 mM molybdate leads to a 4fold increase in activity Thermus thermophilus
1.8.3.1 additional information molybdenum concentrations higher than 2.07 mM do not show increase of enzyme activity Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.1 expressed in Escherichia coli strain TP1000 Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.1 arsenite 7.4 mM, 50% inhibition Thermus thermophilus
1.8.3.1 Ca2+
-
Thermus thermophilus
1.8.3.1 additional information inhibition at 400 mM salt concentrations; no inhibition by periodate or 80 mM sulfate Thermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.3.1 periplasm
-
Thermus thermophilus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.1 K+ 45-100 mM, 54-88% increase of activity Thermus thermophilus
1.8.3.1 Molybdenum the addition of molybdenum to culture media at a concentration of 2.07 mM molybdate leads to a 4fold increase in activity Thermus thermophilus
1.8.3.1 NH4+ 30 mM, 74% increase of activity Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.1 38000
-
gel filtration Thermus thermophilus
1.8.3.1 39100
-
SDS-PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.1 Thermus thermophilus
-
-
-
1.8.3.1 Thermus thermophilus AT62
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.1 DEAE 52 column chromatography, Superdex X26 gel filtration and hydroxyapatite column chromatography Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.3.1 0.191
-
crude cell extract Thermus thermophilus
1.8.3.1 56.67
-
after 297fold purification Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.1 sulfite + H2O + A
-
Thermus thermophilus SO42- + AH2
-
?
1.8.3.1 sulfite + H2O + A
-
Thermus thermophilus AT62 SO42- + AH2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.8.3.1 SO
-
Thermus thermophilus
1.8.3.1 sulfite oxidase
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.3.1 60
-
-
Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.3.1 50 80 The enzyme retains 100% of residual activity up to 7 h of incubation at 50°C. Half life times of the enzyme at 60, 70 and 80°C are respectively 8, 6.5 and 1.5 h. The enzyme retains 30% of activity at 25°C and 45% at 90°C. Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.1 0.4
-
sulfite
-
Thermus thermophilus
1.8.3.1 53.32
-
sulfite
-
Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.1 11
-
-
Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.3.1 9.4
-
Ca2+
-
Thermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.3.1 Thermus thermophilus
-
-
6.8