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Literature summary extracted from

  • Kunz, S.; Kloeckner, T.; Essen, L.O.; Seebeck, T.; Boshart, M.
    TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei (2004), Eur. J. Biochem., 271, 637-647.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.53 TbPDE1 is expressed in Saccharomyces cerevisiae at levels that are sufficient to produce a clear phenotype (heat-shock resistance, growth as a smooth suspension), but that are too low to be detectable in PDE assay of cell lysates. The N-terminally truncated construct pET-PDE1-(Arg189-Thr620) is expressed in Escherichia coli as an active enzyme Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.53 dipyridamole IC50: 0.013 mM Trypanosoma brucei
3.1.4.53 etazolate IC50: 0.025 mM Trypanosoma brucei
3.1.4.53 ethaverine IC50: 0.008 mM Trypanosoma brucei
3.1.4.53 papaverine IC50: 0.03 mM Trypanosoma brucei
3.1.4.53 rolipram IC50: 0.28 mM Trypanosoma brucei
3.1.4.53 sildenafil IC50: 0.001 mM Trypanosoma brucei
3.1.4.53 trequinsin IC50: 0.0025 mM Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.53 Trypanosoma brucei
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.53 bloodstream form
-
 Trypanosoma brucei
-
3.1.4.53 procyclic form
-
 Trypanosoma brucei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.53 3',5'-cAMP + H2O specific for cAMP Trypanosoma brucei 5'-AMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.53 TbPDE1
-
 Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.53 0.001
-
 IC50: 0.001 mM Trypanosoma brucei sildenafil
3.1.4.53 0.0025
-
 IC50: 0.0025 mM Trypanosoma brucei trequinsin
3.1.4.53 0.008
-
 IC50: 0.008 mM Trypanosoma brucei ethaverine
3.1.4.53 0.013
-
 IC50: 0.013 mM Trypanosoma brucei dipyridamole
3.1.4.53 0.025
-
 IC50: 0.025 mM Trypanosoma brucei etazolate
3.1.4.53 0.03
-
 IC50: 0.03 mM Trypanosoma brucei papaverine
3.1.4.53 0.28
-
 IC50: 0.28 mM Trypanosoma brucei rolipram