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Literature summary extracted from

  • Podmore, A.H.B.; Reynolds, P.E.
    Purification and characterization of VanXYc, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resisitant Enterococcus gallinarium BM4174 (2002), Eur. J. Biochem., 269, 2740-2746.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.13.22 D59A 73% decrease in ratio kcat/KM for D-Ala-D-Ala substrate Enterococcus gallinarum
3.4.13.22 D59S 50% increase in ratio kcat/KM for D-Ala-D-Ala substrate Enterococcus gallinarum
3.4.17.8 D59A 73% decrease in ratio kcat/KM for D-Ala-D-Ala substrate, EC3.4.13.B1 activity Enterococcus gallinarum
3.4.17.8 D59S strong increase in ratio kcat/KM for EC 3.4.17.8 activity, 50% increase in ratio kcat/KM for D-Ala-D-Ala substrate, EC3.4.13.B1 activity Enterococcus gallinarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.22 Ni2+ 3.3 mM, no residual activity Enterococcus gallinarum
3.4.13.22 Zn2+ 1.3 mM, 50% inhibition Enterococcus gallinarum
3.4.17.8 D-Ala-D-Ser 30% inhibition Enterococcus gallinarum
3.4.17.8 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-serine 30% inhibition Enterococcus gallinarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.22 2.5
-
 D-Ala-D-Ala mutant D59S, pH 7.5, 37°C Enterococcus gallinarum
3.4.13.22 3.6
-
 D-Ala-D-Ala wild-type, pH 7.5, 37°C Enterococcus gallinarum
3.4.13.22 9
-
 D-Ala-D-Ala mutant D59A, pH 7.5, 37°C Enterococcus gallinarum
3.4.13.22 15.5
-
 D-Ala-D-Ser wild-type, pH 7.5, 37°C Enterococcus gallinarum
3.4.17.8 1.5
-
 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine mutant D59S, pH 7.5, 37°C Enterococcus gallinarum
3.4.17.8 18.8
-
 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine wild-type, pH 7.5, 37°C Enterococcus gallinarum

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.22 Enterococcus gallinarum
-
 bifunctional enzyme with activities of EC 3.4.17.8 and 3.4.13.22
-
3.4.13.22 Enterococcus gallinarum BM4174
-
 bifunctional enzyme with activities of EC 3.4.17.8 and 3.4.13.22
-
3.4.17.8 Enterococcus gallinarum
-
 bifunctional enzyme with activities of EC 3.4.17.8 and 3.4.13.22
-
3.4.17.8 Enterococcus gallinarum BM4174
-
 bifunctional enzyme with activities of EC 3.4.17.8 and 3.4.13.22
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.22 D-Ala-D-Ala + H2O
-
 Enterococcus gallinarum D-Ala + D-Ala
-
 ?
3.4.13.22 D-Ala-D-Ala + H2O
-
 Enterococcus gallinarum BM4174 D-Ala + D-Ala
-
 ?
3.4.13.22 D-Ala-D-Ser + H2O
-
 Enterococcus gallinarum D-Ala + D-Ser
-
 ?
3.4.13.22 D-Ala-D-Ser + H2O
-
 Enterococcus gallinarum BM4174 D-Ala + D-Ser
-
 ?
3.4.17.8 additional information no substrate: UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-serine Enterococcus gallinarum ?
-
 ?
3.4.17.8 additional information no substrate: UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-serine Enterococcus gallinarum BM4174 ?
-
 ?
3.4.17.8 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
-
 Enterococcus gallinarum UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanine + D-alanine
-
 ?
3.4.17.8 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
-
 Enterococcus gallinarum BM4174 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanine + D-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.22 VanXYc
-
 Enterococcus gallinarum
3.4.17.8 VanXYc
-
 Enterococcus gallinarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.22 0.35
-
 D-Ala-D-Ser wild-type, pH 7.5, 37°C Enterococcus gallinarum
3.4.13.22 0.8
-
 D-Ala-D-Ala mutant D59A, pH 7.5, 37°C Enterococcus gallinarum
3.4.13.22 1.4
-
 D-Ala-D-Ala mutant D59S, pH 7.5, 37°C Enterococcus gallinarum
3.4.13.22 2.5
-
 D-Ala-D-Ala wild-type, pH 7.5, 37°C Enterococcus gallinarum
3.4.17.8 6.2
-
 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine wild-type, pH 7.5, 37°C Enterococcus gallinarum
3.4.17.8 23
-
 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine mutant D59S, pH 7.5, 37°C Enterococcus gallinarum