EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 0.14 | - |
methyl caffeate | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 0.57 | - |
methyl sinapinate | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 0.57 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 0.59 | - |
methyl p-coumarate | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 0.62 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 1.64 | - |
methyl ferulate | - |
Thermothelomyces heterothallicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.73 | extracellular | - |
Thermothelomyces heterothallicus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 23000 | - |
2 * 23000, SDS-PAGE, native mass by native PAGE | Thermothelomyces heterothallicus |
3.1.1.73 | 43000 | - |
native PAGE | Thermothelomyces heterothallicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | feruloyl polysaccharide + H2O | Thermothelomyces heterothallicus | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
3.1.1.73 | wheat bran + H2O | Thermothelomyces heterothallicus | - |
ferulic acid + ? | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.73 | additional information | activity is retained in hexane/1-butanol/water surfactantless microemulsion | Thermothelomyces heterothallicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Thermothelomyces heterothallicus | - |
strain ATCC 34628, feruloyl esterase type C | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.73 | - |
Thermothelomyces heterothallicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 3.1 | - |
purified enzyme | Thermothelomyces heterothallicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | C-5 linked ferulate is hydrolyzed three times more efficiently than C-2 linked ferulate | Thermothelomyces heterothallicus | ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside | - |
? | |
3.1.1.73 | 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | C-5 linked ferulate is hydrolyzed three times more efficiently than C-2 linked ferulate | Thermothelomyces heterothallicus | ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside | - |
? | |
3.1.1.73 | feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Thermothelomyces heterothallicus | ferulic acid + ? | - |
? | |
3.1.1.73 | methyl caffeate + H2O | - |
Thermothelomyces heterothallicus | caffeic acid + methanol | - |
? | |
3.1.1.73 | methyl ferulate + H2O | - |
Thermothelomyces heterothallicus | ferulic acid + methanol | - |
? | |
3.1.1.73 | methyl p-coumarate + H2O | - |
Thermothelomyces heterothallicus | p-coumaric acid + methanol | - |
? | |
3.1.1.73 | methyl sinapinate + H2O | - |
Thermothelomyces heterothallicus | sinapic acid + methanol | - |
? | |
3.1.1.73 | wheat bran + H2O | - |
Thermothelomyces heterothallicus | ferulic acid + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.73 | dimer | 2 * 23000, SDS-PAGE, native mass by native PAGE | Thermothelomyces heterothallicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.73 | StFaeC | - |
Thermothelomyces heterothallicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 55 | - |
- |
Thermothelomyces heterothallicus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 60 | - |
82% of activity | Thermothelomyces heterothallicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 0.1 | - |
methyl caffeate | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 0.4 | - |
methyl sinapinate | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 0.5 | - |
methyl p-coumarate | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 2.6 | - |
methyl ferulate | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 3.7 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Thermothelomyces heterothallicus | |
3.1.1.73 | 10.3 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Thermothelomyces heterothallicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 6 | - |
- |
Thermothelomyces heterothallicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 8 | - |
80% of activity | Thermothelomyces heterothallicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 6 | 10 | stable, 80% of activity after 24 h at pH 10 | Thermothelomyces heterothallicus |
3.1.1.73 | 10 | - |
half life at 55°C: 133 min, half life at 60°C: 55 min | Thermothelomyces heterothallicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.73 | Thermothelomyces heterothallicus | isoelectric focusing and cation-exchange chromatofocusing | - |
3.1 |