Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Topakas, E.; Vafiadi, C.; Stamatis, H.; Christakopoulos, P.
    Sporotrichum thermophile type C feruloyl esterase (StFaeC): purification, characterization, and its use for phenolic acid (sugar) ester synthesis (2005), Enzyme Microb. Technol., 36, 729-736.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.14
-
methyl caffeate
-
Thermothelomyces heterothallicus
3.1.1.73 0.57
-
methyl sinapinate
-
Thermothelomyces heterothallicus
3.1.1.73 0.57
-
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside
-
Thermothelomyces heterothallicus
3.1.1.73 0.59
-
methyl p-coumarate
-
Thermothelomyces heterothallicus
3.1.1.73 0.62
-
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside
-
Thermothelomyces heterothallicus
3.1.1.73 1.64
-
methyl ferulate
-
Thermothelomyces heterothallicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
Thermothelomyces heterothallicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 23000
-
2 * 23000, SDS-PAGE, native mass by native PAGE Thermothelomyces heterothallicus
3.1.1.73 43000
-
native PAGE Thermothelomyces heterothallicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl polysaccharide + H2O Thermothelomyces heterothallicus involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
?
3.1.1.73 wheat bran + H2O Thermothelomyces heterothallicus
-
ferulic acid + ?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.73 additional information activity is retained in hexane/1-butanol/water surfactantless microemulsion Thermothelomyces heterothallicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Thermothelomyces heterothallicus
-
strain ATCC 34628, feruloyl esterase type C
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
Thermothelomyces heterothallicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 3.1
-
purified enzyme Thermothelomyces heterothallicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O C-5 linked ferulate is hydrolyzed three times more efficiently than C-2 linked ferulate Thermothelomyces heterothallicus ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
?
3.1.1.73 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O C-5 linked ferulate is hydrolyzed three times more efficiently than C-2 linked ferulate Thermothelomyces heterothallicus ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
?
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Thermothelomyces heterothallicus ferulic acid + ?
-
?
3.1.1.73 methyl caffeate + H2O
-
Thermothelomyces heterothallicus caffeic acid + methanol
-
?
3.1.1.73 methyl ferulate + H2O
-
Thermothelomyces heterothallicus ferulic acid + methanol
-
?
3.1.1.73 methyl p-coumarate + H2O
-
Thermothelomyces heterothallicus p-coumaric acid + methanol
-
?
3.1.1.73 methyl sinapinate + H2O
-
Thermothelomyces heterothallicus sinapic acid + methanol
-
?
3.1.1.73 wheat bran + H2O
-
Thermothelomyces heterothallicus ferulic acid + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 dimer 2 * 23000, SDS-PAGE, native mass by native PAGE Thermothelomyces heterothallicus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 StFaeC
-
Thermothelomyces heterothallicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 55
-
-
Thermothelomyces heterothallicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 60
-
82% of activity Thermothelomyces heterothallicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.1
-
methyl caffeate
-
Thermothelomyces heterothallicus
3.1.1.73 0.4
-
methyl sinapinate
-
Thermothelomyces heterothallicus
3.1.1.73 0.5
-
methyl p-coumarate
-
Thermothelomyces heterothallicus
3.1.1.73 2.6
-
methyl ferulate
-
Thermothelomyces heterothallicus
3.1.1.73 3.7
-
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside
-
Thermothelomyces heterothallicus
3.1.1.73 10.3
-
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside
-
Thermothelomyces heterothallicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6
-
-
Thermothelomyces heterothallicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 8
-
80% of activity Thermothelomyces heterothallicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 6 10 stable, 80% of activity after 24 h at pH 10 Thermothelomyces heterothallicus
3.1.1.73 10
-
half life at 55°C: 133 min, half life at 60°C: 55 min Thermothelomyces heterothallicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Thermothelomyces heterothallicus isoelectric focusing and cation-exchange chromatofocusing
-
3.1