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Literature summary extracted from
- A novel penicillin acylase from the environmental gene pool with improved synthetic properties (2005), Enzyme Microb. Technol., 36, 182-190.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.11 | 0.004 | - |
6-nitro-3-[(phenylacetyl)amino]benzoic acid | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium |  |
| 3.5.1.11 | 0.012 | - |
penicillin G | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 0.013 | - |
penicillin G | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 0.015 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 0.027 | - |
4-Hydroxyphenylacetamide | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 0.03 | - |
phenylacetamide | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 0.114 | - |
4-Hydroxyphenylacetamide | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 0.156 | - |
phenylacetamide | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 0.284 | - |
cefadroxil | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 0.399 | - |
amoxicillin | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 0.575 | - |
ampicillin | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 0.646 | - |
cefadroxil | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 0.646 | - |
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 1.07 | - |
amoxicillin | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 1.3 | - |
cephalexin | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 1.3 | - |
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 1.5 | - |
cephalexin | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 2.5 | - |
ampicillin | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 9.1 | - |
D-4-hydroxyphenylglycine amide | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 12 | - |
D-phenylglycine amide | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 12.2 | - |
D-4-hydroxyphenylglycine amide | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 30 | - |
D-phenylglycine amide | pH 7.0, 30°C | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 25500 | - |
x * 25500, alpha-subunit, + x * 61900, beta-subunit, SDS-PAGE | uncultured Gammaproteobacteria bacterium |
| 3.5.1.11 | 61900 | - |
x * 25500, alpha-subunit, + x * 61900, beta-subunit, SDS-PAGE | uncultured Gammaproteobacteria bacterium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.11 | Escherichia coli | P06875 | - |
- |
| 3.5.1.11 | uncultured Gammaproteobacteria bacterium | Q6PWR5 | TaxID 86473, growth on D-phenylglycine as sole source of nitrogen | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.11 | -20°C, enzyme may be defrosted several times without loss of activity | uncultured Gammaproteobacteria bacterium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | 4-hydroxyphenylacetamide + 6-aminopenicillanic acid | - |
uncultured Gammaproteobacteria bacterium | ? | - |
r | |
| 3.5.1.11 | 4-hydroxyphenylacetamide + 6-aminopenicillanic acid | - |
Escherichia coli | ? | - |
r | |
| 3.5.1.11 | 6-aminopenicillanate + phenylacetic acid | - |
Escherichia coli | penicillin G + H2O | - |
r | |
| 3.5.1.11 | 6-aminopenicillanate + phenylacetic acid | synthesis of penicillin G by enzyme is significantly better than by Escherichia coli enzyme | uncultured Gammaproteobacteria bacterium | penicillin G + H2O | - |
r | |
| 3.5.1.11 | 6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O | - |
uncultured Gammaproteobacteria bacterium | 3-amino-6-nitrobenzoic acid + phenylacetic acid | - |
r | |
| 3.5.1.11 | 6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O | - |
Escherichia coli | 3-amino-6-nitrobenzoic acid + phenylacetic acid | - |
r | |
| 3.5.1.11 | amoxillin + H2O | - |
uncultured Gammaproteobacteria bacterium | D-4-hydroxyphenylglycine + 6-aminopenicillanic acid | - |
r | |
| 3.5.1.11 | amoxillin + H2O | - |
Escherichia coli | D-4-hydroxyphenylglycine + 6-aminopenicillanic acid | - |
r | |
| 3.5.1.11 | ampicillin + H2O | - |
uncultured Gammaproteobacteria bacterium | (R)-2-phenylglycine + 6-aminopenicillanic acid | - |
r | |
| 3.5.1.11 | ampicillin + H2O | - |
Escherichia coli | (R)-2-phenylglycine + 6-aminopenicillanic acid | - |
r | |
| 3.5.1.11 | cefadroxil + H2O | - |
uncultured Gammaproteobacteria bacterium | ? | - |
r | |
| 3.5.1.11 | cefadroxil + H2O | - |
Escherichia coli | ? | - |
r | |
| 3.5.1.11 | cephalexin + H2O | - |
uncultured Gammaproteobacteria bacterium | 7-aminodesacetoxycephalosporanic acid + D-phenylglycine | - |
r | |
| 3.5.1.11 | cephalexin + H2O | - |
Escherichia coli | 7-aminodesacetoxycephalosporanic acid + D-phenylglycine | - |
r | |
| 3.5.1.11 | D-2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O | - |
uncultured Gammaproteobacteria bacterium | phenylacetic acid + 5-amino-2-nitrobenzoic acid | - |
r | |
| 3.5.1.11 | D-2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O | - |
Escherichia coli | phenylacetic acid + 5-amino-2-nitrobenzoic acid | - |
r | |
| 3.5.1.11 | D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid | - |
uncultured Gammaproteobacteria bacterium | amoxicillin + NH3 | - |
r | |
| 3.5.1.11 | D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid | - |
Escherichia coli | amoxicillin + NH3 | - |
r | |
| 3.5.1.11 | D-phenylglycine amide + 6-aminopenicillanic acid | - |
uncultured Gammaproteobacteria bacterium | ampicillin + NH3 | - |
r | |
| 3.5.1.11 | D-phenylglycine amide + 6-aminopenicillanic acid | - |
Escherichia coli | ampicillin + NH3 | - |
r | |
| 3.5.1.11 | penicillin G + H2O | - |
uncultured Gammaproteobacteria bacterium | 6-aminopenicillanate + phenylacetic acid | - |
r | |
| 3.5.1.11 | penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanate + phenylacetic acid | - |
r | |
| 3.5.1.11 | phenylacetamide + 6-aminopenicillanic acid | - |
uncultured Gammaproteobacteria bacterium | ? | - |
r | |
| 3.5.1.11 | phenylacetamide + 6-aminopenicillanic acid | - |
Escherichia coli | ? | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | ? | x * 25500, alpha-subunit, + x * 61900, beta-subunit, SDS-PAGE | uncultured Gammaproteobacteria bacterium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.11 | 2 | 8 | D-4-hydroxyphenylglycine amide | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | Escherichia coli | |
| 3.5.1.11 | 12 | - |
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 13 | - |
cefadroxil | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 14 | - |
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | Escherichia coli | |
| 3.5.1.11 | 15 | - |
amoxicillin | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 16 | - |
ampicillin | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 16 | - |
D-4-hydroxyphenylglycine amide | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 17 | - |
amoxicillin | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 18 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | Escherichia coli | |
| 3.5.1.11 | 20 | - |
cephalexin | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 23 | - |
phenylacetamide | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 24 | - |
6-nitro-3-[(phenylacetyl)amino]benzoic acid | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 25 | - |
penicillin G | pH 7.0, 30°C | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 25 | - |
ampicillin | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 25 | - |
D-phenylglycine amide | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 29 | - |
cephalexin | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 29 | - |
4-Hydroxyphenylacetamide | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | uncultured Gammaproteobacteria bacterium | |
| 3.5.1.11 | 32 | - |
cefadroxil | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 39 | - |
penicillin G | pH 7.0, 30°C | Escherichia coli | |
| 3.5.1.11 | 46 | - |
phenylacetamide | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | Escherichia coli | |
| 3.5.1.11 | 47 | - |
4-Hydroxyphenylacetamide | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | Escherichia coli | |
| 3.5.1.11 | 57 | - |
D-phenylglycine amide | pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma | Escherichia coli | |
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