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Literature summary extracted from

  • De Azeredo, L.A.; Freire, D.M.; Soares, R.M.; Leite, S.G.; Coelho, R.R.
    Production and partial characterization of thermophilic proteases from Streptomyces sp. isolated from Brazilian cerrado soil (2004), Enzyme Microb. Technol., 34, 354-358.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.24.77 degradation enzymatic and microbiological hydrolysis at the industrial level Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.77 Cu2+ 10 mM, 46% inhibition Streptomyces sp.
3.4.24.77 EDTA 10 mM, 74% inhibition Streptomyces sp.
3.4.24.77 Phenanthroline
-
 Streptomyces sp.
3.4.24.77 phenyl-methyl-sulfonyl-fluoride
-
 Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.77 Ba2+ 10 mM, 1.37fold activity enhancement after 1 h at 55°C Streptomyces sp.
3.4.24.77 Ca2+ 10 mM, 1.24fold activity enhancement after 1 h at 55°C Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.77 Streptomyces sp.
-
-
-
3.4.24.77 Streptomyces sp. 594
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.4.24.77 -20°C, crude enzyme preparation, 2 months, 25% loss of activity Streptomyces sp.
3.4.24.77 4°C, crude enzyme preparation, 2 months, 25% loss of activity Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.77 azocasein + H2O
-
 Streptomyces sp. ?
-
 ?
3.4.24.77 azocasein + H2O
-
 Streptomyces sp. 594 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.77 thermophilic protease
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.77 55 70
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.77 30 40 the enzyme retains 90% of activity at 30°C and 40°C after 1 h incubation Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.77 6
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.77 5 8
-
 Streptomyces sp.