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Literature summary extracted from

  • Lakhman, S.S.; Ghosh, D.; Blanco, J.G.
    Functional significance of a natural allelic variant of human carbonyl reductase 3 (CBR3) (2005), Drug Metab. Dispos., 33, 254-257.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 isozyme CBR3 V244M, genotyping and polymorphism distribution in 10 different ethnic groups, overview, expression of isozymes CBR V244 and CBR M244 in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.184 V244M naturally occuring polymorphism in isozyme CBR3, the isozyme CBR M244 may reflect a faster association-dissociation rate for NADPH/NADP+ per catalytic cycle, residue 244 is not involved in direct cofactor interaction, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.0229
-
 menadione pH 7.4, 37°C, recombinant isozyme CBR M244 Homo sapiens
1.1.1.184 0.0246
-
 menadione pH 7.4, 37°C, recombinant isozyme CBR V244 Homo sapiens
1.1.1.184 0.085
-
 NADPH pH 7.4, 37°C, recombinant isozyme CBR M244 Homo sapiens
1.1.1.184 0.0905
-
 NADPH pH 7.4, 37°C, recombinant isozyme CBR V244 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Homo sapiens
-
 isozyme CBR3
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 menadione + NADPH + H+
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 More partial structure modeling Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 carbonyl reductase 3
-
 Homo sapiens
1.1.1.184 CBR3
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.184 9.9
-
 menadione pH 7.4, 37°C, recombinant isozyme CBR V244 Homo sapiens
1.1.1.184 16.3
-
 NADPH pH 7.4, 37°C, recombinant isozyme CBR V244 Homo sapiens
1.1.1.184 20.5
-
 menadione pH 7.4, 37°C, recombinant isozyme CBR M244 Homo sapiens
1.1.1.184 26.7
-
 NADPH pH 7.4, 37°C, recombinant isozyme CBR M244 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH binding site modeling Homo sapiens