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Literature summary extracted from

  • Swenson, S.; Toombs, C.F.; Pena, L.; Johansson, J.; Markland, F.S.
    alpha-Fibrinogenases (2004), Curr. Drug Targets Cardiovasc. Haematol. Disord., 4, 417-435.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.72 1,10-phenanthroline complete and rapid inhibition Agkistrodon contortrix
3.4.24.72 alpha2-Macroglobulin
-
 Agkistrodon contortrix
3.4.24.72 EDTA complete and rapid inhibition Agkistrodon contortrix
3.4.24.72 Tetraethylenepentamine complete and rapid inhibition Agkistrodon contortrix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.72 0.000000242
-
 low molecular weight kininogen
-
 Agkistrodon contortrix
3.4.24.72 0.00000113
-
 His-Thr-Glu-Phe-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000138
-
 Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val
-
 Agkistrodon contortrix
3.4.24.72 0.00000164
-
 His-Thr-Glu-Glu-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000247
-
 His-Thr-Glu-Lys-Phe-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000338
-
 His-Thr-Glu-Arg-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000391
-
 His-Thr-Glu-Pro-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000415
-
 His-Thr-Glu-His-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000528
-
 His-Thr-Glu-Leu-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000579
-
 His-Thr-Glu-Lys-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000739
-
 His-Thr-Glu-Ala-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000832
-
 His-Thr-Glu-Ser-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.00000937
-
 His-Thr-Glu-Asn-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.0000654
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Agkistrodon contortrix
3.4.24.72 0.000579
-
 His-Thr-Glu-Lys-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.72 22890
-
 calculation from amino acid sequence Agkistrodon contortrix
3.4.24.72 60000
-
 polyethylene-glycosylated enzyme Agkistrodon contortrix

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Agkistrodon contortrix
-
 subspecies Agkistrodon contortrix contortrix
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.72 glycoprotein adduction of polyethylene glycol to reduce the rate of clearance from the circulation, and coupling to an Arg-Gly-Asp (RGD) like peptide imparting inhibitory activity on platelet aggregation and thrombus formation while maintaining full fibrinolytic activity Agkistrodon contortrix

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72 high performance liquid chromatography, hydroxyapatite high performance liquid chromatography and cation exchange high performance liquid chromatography Agkistrodon contortrix

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.72 venom
-
 Agkistrodon contortrix
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
-
 Agkistrodon contortrix Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg
-
 ir
3.4.24.72 azocasein + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 Fibrin + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 Fibrinogen + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Ala-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Arg-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Asn-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Glu-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-His-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Leu-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-Ala-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-Arg-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-Asn-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-Glu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-His-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-Phe-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-Pro-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Lys-Ser-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Phe-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Pro-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 His-Thr-Glu-Ser-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val + H2O
-
 Agkistrodon contortrix ?
-
 ?
3.4.24.72 low molecular weight kininogen + H2O
-
 Agkistrodon contortrix kallidin + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.72 fibrinolytic protease
-
 Agkistrodon contortrix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.72 additional information
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Agkistrodon contortrix
3.4.24.72 0.0086
-
 low molecular weight kininogen
-
 Agkistrodon contortrix
3.4.24.72 0.09
-
 Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val
-
 Agkistrodon contortrix
3.4.24.72 0.16
-
 His-Thr-Glu-Leu-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.2
-
 His-Thr-Glu-Lys-Phe-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.45
-
 His-Thr-Glu-Phe-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 0.46
-
 His-Thr-Glu-Glu-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 1.59
-
 His-Thr-Glu-Pro-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 1.97
-
 His-Thr-Glu-Arg-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 2.23
-
 His-Thr-Glu-Lys-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 3.98
-
 His-Thr-Glu-Ser-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 5
-
 His-Thr-Glu-Ala-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 5.19
-
 His-Thr-Glu-His-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.4.24.72 6.03
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Agkistrodon contortrix
3.4.24.72 65.7
-
 His-Thr-Glu-Asn-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.72 7.4
-
-
 Agkistrodon contortrix

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.24.72 Agkistrodon contortrix
-
-
 6.8