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Literature summary extracted from

  • von Elert, E.; Agrawal, M.K.; Gebauer, C.; Jaensch, H.; Bauer, U.; Zitt, A.
    Protease activity in gut of Daphnia magna: evidence for trypsin and chymotrypsin enzymes (2004), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 137, 287-296.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.1 chymostatin strong inhibitory effect in concentrations starting from 0.001 mM, 50% inhibition by 0.05 mM Daphnia magna
3.4.21.1 additional information no inhibition by N-p-tosyl-lysine chloroketone and 4-(amidinopheyl)methanesulfonyl fluoride Daphnia magna
3.4.21.4 4-(amidinophenyl)methanesulfonyl fluoride
-
 Daphnia magna
3.4.21.4 additional information not inhibitory: chymostatin Daphnia magna
3.4.21.4 N-p-tosyl-lysine chloroketone
-
 Daphnia magna

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.1 Daphnia magna
-
-
-
3.4.21.4 Daphnia magna
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.1 gut
-
 Daphnia magna
-
3.4.21.4 gut
-
 Daphnia magna
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.1 additional information
-
 no hydrolysis of N-benzoyl-L-tyrosine p-nitroanilide Daphnia magna
3.4.21.1 0.3221
-
 N-succinyl-L-alanyl-L-alanyl-L-prolinyl-L-phenylalanine p-nitroanilide Daphnia magna

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.1 azocasein + H2O
-
 Daphnia magna ?
-
 ?
3.4.21.1 N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine p-nitroanilide + H2O
-
 Daphnia magna p-nitroaniline + N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine
-
 ?
3.4.21.4 N-benzoyl-DL-Arg-4-nitroanilide + H2O
-
 Daphnia magna N-benzoyl-DL-Arg + 4-nitroaniline
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.1 7 10
-
 Daphnia magna

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.1 7 10
-
 Daphnia magna
3.4.21.4 7 10
-
 Daphnia magna