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Literature summary extracted from

  • Glowacka, A.E.; Podstawka, E.; Szczesna-Antczak, M.H.; Kalinowska, H.; Antczak, T.
    Kinetic and molecular properties of Bacillus subtilis IBTC-3 subtilisin (2005), Comp. Biochem. Physiol. B, 140, 321-331.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.62 0.09
-
 Urea-denatured hemoglobin 30°C, pH 8.0 Bacillus subtilis
3.4.21.62 0.22
-
 acetyl-L-Phe ethyl ester 30°C, pH 8.0 Bacillus subtilis
3.4.21.62 0.37
-
 acetyl-L-Tyr ethyl ester 30°C, pH 8.0 Bacillus subtilis
3.4.21.62 0.85
-
 acetyl-L-Phe 30°C, pH 8.0 Bacillus subtilis
3.4.21.62 2.1
-
 benzoyl-L-Arg ethyl ester 30°C, pH 8.0 Bacillus subtilis
3.4.21.62 4
-
 acetyl-L-Tyr 30°C, pH 8.0 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.62 27000
-
 x * 27000, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus subtilis
-
-
-
3.4.21.62 Bacillus subtilis IBTC-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.62
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.62 28.1
-
 pH 8.0, 30°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 acetyl-L-Phe + ethanol
-
 Bacillus subtilis acetyl-L-Phe ethyl ester + H2O
-
 r
3.4.21.62 acetyl-L-Phe + ethanol
-
 Bacillus subtilis IBTC-3 acetyl-L-Phe ethyl ester + H2O
-
 r
3.4.21.62 acetyl-L-Phe ethyl ester + H2O
-
 Bacillus subtilis acetyl-L-Phe + ethanol
-
 r
3.4.21.62 acetyl-L-Phe ethyl ester + H2O
-
 Bacillus subtilis IBTC-3 acetyl-L-Phe + ethanol
-
 r
3.4.21.62 acetyl-L-Tyr + ethanol
-
 Bacillus subtilis acetyl-L-Tyr ethyl ester + H2O
-
 r
3.4.21.62 acetyl-L-Tyr ethyl ester + H2O
-
 Bacillus subtilis acetyl-L-Tyr + ethanol
-
 r
3.4.21.62 acetyl-L-Tyr ethyl ester + H2O
-
 Bacillus subtilis IBTC-3 acetyl-L-Tyr + ethanol
-
 r
3.4.21.62 benzoyl-L-Arg + ethanol
-
 Bacillus subtilis benzoyl-L-Arg ethyl ester + H2O
-
 r
3.4.21.62 Benzoyl-L-Arg ethyl ester + H2O
-
 Bacillus subtilis Benzoyl-L-Arg + ethanol
-
 r
3.4.21.62 Benzoyl-L-Arg ethyl ester + H2O
-
 Bacillus subtilis IBTC-3 Benzoyl-L-Arg + ethanol
-
 r
3.4.21.62 Urea-denatured hemoglobin + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.21.62 Urea-denatured hemoglobin + H2O
-
 Bacillus subtilis IBTC-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.62 ? x * 27000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.62 60
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.62 30
-
 about 40% of maximum activity Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.62 0.022
-
 acetyl-L-Phe 30°C, pH 8.0 Bacillus subtilis
3.4.21.62 0.04
-
 acetyl-L-Tyr ethyl ester 30°C, pH 8.0 Bacillus subtilis
3.4.21.62 0.04
-
 Urea-denatured hemoglobin 30°C, pH 8.0 Bacillus subtilis
3.4.21.62 0.1
-
 acetyl-L-Tyr 30°C, pH 8.0 Bacillus subtilis
3.4.21.62 0.38
-
 acetyl-L-Phe ethyl ester 30°C, pH 8.0 Bacillus subtilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.62 Bacillus subtilis isoelectric focusing
-
 8.4