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Literature summary extracted from

  • Luo, L.Y.; Shan, S.J.; Elliott, M.B.; Soosaipillai, A.; Diamandis, E.P.
    Purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma (2006), Clin. Cancer Res., 12, 742-750.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.35 (4-amidino-phenyl)-methane-sulfonyl fluoride 2.5 mM, complete inhibition Homo sapiens
3.4.21.35 Aprotinin up to 40% inhibition Homo sapiens
3.4.21.35 hK11-specific monoclonal antibody up to 40% inhibition Homo sapiens
3.4.21.B42 amidinophenylmethansulfonylfluorid complete inhibition at 2.5 microM Homo sapiens
3.4.21.B42 Aprotinin 30% inhibition at 2.5 mM Homo sapiens
3.4.21.B42 benzamidine 40% inhibition at 2.5 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.35 0.27
-
 Gln-Ala-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.35 0.28
-
 Val-Pro-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.35 0.44
-
 Phe-Ser-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.35 0.53
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.35 1.18
-
 Gly-Pro-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.B42 0.27
-
 Gln-Ala-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C Homo sapiens
3.4.21.B42 0.28
-
 Val-Pro-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C Homo sapiens
3.4.21.B42 0.44
-
 Phe-Ser-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C Homo sapiens
3.4.21.B42 0.53
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C Homo sapiens
3.4.21.B42 1.18
-
 Gly-Pro-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.35 additional information
-
 about 40% of human tissue kallikrein 11 (40000 Da) is enzymatically active, whereas the rest is inactivated by internal cleavage after Arg156 which generates two peptides of 20000 Da, connected by internal disulfide bonds Homo sapiens
3.4.21.35 40000
-
 x * 40000, SDS-PAGE Homo sapiens
3.4.21.B42 40000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.35 Homo sapiens Q9UBX7
-
-
3.4.21.B42 Homo sapiens Q9UBX7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.35
-
 Homo sapiens
3.4.21.B42 to homogeneity by immunoaffinity chromatography and high-performance liquid chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.35 seminal plasma
-
 Homo sapiens
-
3.4.21.B42 seminal plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.35 Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 additional information the enzyme cleaves synthetic peptides after arginine but not lysine residues. It does not cleave chymotrypsin Homo sapiens ?
-
 ?
3.4.21.35 Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B42 Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B42 Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B42 Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B42 Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B42 Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.35 ? x * 40000, SDS-PAGE Homo sapiens
3.4.21.B42 monomer SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.35 hK11
-
 Homo sapiens
3.4.21.35 KLK11
-
 Homo sapiens
3.4.21.35 tissue kallikrein 11
-
 Homo sapiens
3.4.21.B42 hK11
-
 Homo sapiens
3.4.21.B42 human kallikrein 11
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.35 0.0035
-
 Gly-Pro-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.35 0.0038
-
 Gln-Ala-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.35 0.0065
-
 Phe-Ser-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.35 0.0075
-
 Val-Pro-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.35 0.031
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
3.4.21.B42 0.21
-
 Gly-Pro-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C Homo sapiens
3.4.21.B42 0.23
-
 Gln-Ala-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C Homo sapiens
3.4.21.B42 0.39
-
 Phe-Ser-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C Homo sapiens
3.4.21.B42 0.45
-
 Val-Pro-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C Homo sapiens
3.4.21.B42 1.86
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin 20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.35 7.5
-
 hydrolysis of Pro-Phe-Arg-7-amido-4-methylcoumarin Homo sapiens
3.4.21.B42 7.5
-
-
 Homo sapiens