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Literature summary extracted from

  • Ocadiz, R.; Orozco, E.; Carrillo, E.; Quintas, L.I.; Ortega-Lopez, J.; Garcia-Perez, R.M.; Sanchez, T.; Castillo-Juarez, B.A.; Garcia-Rivera, G.; Rodriguez, M.A.
    EhCP112 is an Entamoeba histolytica secreted cysteine protease that may be involved in the parasite-virulence (2005), Cell. Microbiol., 7, 221-232.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.35 gene ehcp112, functional expression of the His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, in Escherichia coli strain BL21 Entamoeba histolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.35 extracellular EhCP112 is secreted Entamoeba histolytica
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.35 52000
-
 x * 52000, recombinant His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, SDS-PAGE Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.35 collagen type 1 + H2O Entamoeba histolytica
-
 ?
-
 ?
3.4.22.35 Fibronectin + H2O Entamoeba histolytica
-
 ?
-
 ?
3.4.22.35 additional information Entamoeba histolytica the enzyme is involved in host tissue invasion by trophozoites and destroys cell monolayers ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.35 Entamoeba histolytica
-
 gene ehcp112
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.35 recombinant His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, from Escherichia coli strain BL21 by nickel affinity chromatography Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.35 trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.35 azocasein + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 collagen type 1 + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 Fibronectin + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 additional information the enzyme is involved in host tissue invasion by trophozoites and destroys cell monolayers Entamoeba histolytica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.35 ? x * 52000, recombinant His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, SDS-PAGE Entamoeba histolytica
3.4.22.35 More the enzyme contains an RDG domain Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
3.4.22.35 EhCP112
-
 Entamoeba histolytica