EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.184 | expression in Escherichia coli strain BL21(DE3) | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.184 | rutin | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.184 | additional information | - |
additional information | recombinant enzyme, steady-state kinetics, molecular modeling and substrate docking studies | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.184 | daunorubicin + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | daunorubicinol + NADP+ | - |
? | |
1.1.1.184 | doxorubicin + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | doxorubicinol + NADP+ | - |
? | |
1.1.1.184 | menadione + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | ? | - |
? | |
1.1.1.184 | mitroxantrone + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | ? | - |
? | |
1.1.1.184 | naphthazarin + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.184 | Homo sapiens | P16152 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.184 | recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.184 | R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ | molecular modeling and enzyme-substrate docking studies, reaction mechanism with anthracyclin substrates | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.184 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.184 | 13-deoxydoxorubicin + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | ? | - |
? | |
1.1.1.184 | daunorubicin + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | daunorubicinol + NADP+ | - |
? | |
1.1.1.184 | daunorubicin + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | daunorubicinol + NADP+ | - |
? | |
1.1.1.184 | doxorubicin + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | doxorubicinol + NADP+ | - |
? | |
1.1.1.184 | doxorubicin + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | doxorubicinol + NADP+ | - |
? | |
1.1.1.184 | menadione + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | ? | - |
? | |
1.1.1.184 | menadione + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | ? | - |
? | |
1.1.1.184 | mitroxantrone + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | ? | - |
? | |
1.1.1.184 | mitroxantrone + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | ? | - |
? | |
1.1.1.184 | naphthazarin + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | ? | - |
? | |
1.1.1.184 | naphthazarin + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.184 | More | molecular modeling and generation of a three-dimensional structural model | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.184 | carbonyl reductase 1 | - |
Homo sapiens |
1.1.1.184 | CBR1 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.184 | 25 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.184 | 7.5 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.184 | NADPH | dependent on | Homo sapiens |