BRENDA - Enzyme Database

Comparison of Mycobacterium tuberculosis isocitrate dehydrogenases (ICD-1 and ICD-2) reveals differences in coenzyme affinity, oligomeric state, pH tolerance and phylogenetic affiliation

Banerjee, S.; Nandyala, A.; Podili, R.; Katoch, V.M.; Hasnain, S.E.; BMC Biochem. 6, 20 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.42
NaCl
isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory
Mycobacterium tuberculosis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
NaCl
isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory
Mycobacterium tuberculosis
1.1.1.42
NADPH
isoform ICD-2, competitive inhibition
Mycobacterium tuberculosis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.01
-
DL-isocitrate
isoform ICD-1, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
0.02
-
DL-isocitrate
isoform ICD-2, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
0.022
-
DL-isocitrate
isoform ICD-1, presence of Zn2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
0.125
-
NADP+
isoform ICD-1, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
19.6
-
NADP+
isoform ICD-2, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
both isoforms ICD-1, ICD-2, saturation at 10 mM
Mycobacterium tuberculosis
1.1.1.42
additional information
no acitivty of isoforms with Mn2+ or Ca2+
Mycobacterium tuberculosis
1.1.1.42
Zn2+
isoform ICD-1, can replace Mg2+, saturation at 10 mM
Mycobacterium tuberculosis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
49000
-
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer; 4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1
Mycobacterium tuberculosis
1.1.1.42
86000
-
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer; and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2
Mycobacterium tuberculosis
1.1.1.42
100000
-
gel filtration, main peak, isoform ICD-1
Mycobacterium tuberculosis
1.1.1.42
180000
-
gel filtration, isoform ICD-2
Mycobacterium tuberculosis
1.1.1.42
200000
-
gel filtration, isoform ICD-1
Mycobacterium tuberculosis
1.1.1.42
320000
-
gel filtration, isoform ICD-2
Mycobacterium tuberculosis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Mycobacterium tuberculosis
-
isoforms ICD-1 and ICD-2
-
Renatured (Commentary)
EC Number
Commentary
Organism
1.1.1.42
isoform ICD-2, renaturation of protein inactivated for 30 min at 65C by slow cooling till 55C
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
-
668080
Mycobacterium tuberculosis
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer
Mycobacterium tuberculosis
1.1.1.42
tetramer
4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1
Mycobacterium tuberculosis
1.1.1.42
trimer
and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2
Mycobacterium tuberculosis
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
20
40
isoform ICD-2
Mycobacterium tuberculosis
1.1.1.42
40
-
isoform ICD-1
Mycobacterium tuberculosis
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.1.1.42
60
-
isoform ICD-1, 40% residual activity, ICD-2, 5% residual activity
Mycobacterium tuberculosis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.42
65
-
30 min, isoform ICD-1, irreversible thermal inactivation, isoform ICD-2, renaturation of inactivated protein by slow cooling till 55C
Mycobacterium tuberculosis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.42
1.9
-
DL-isocitrate
isoform ICD-1, presence of Zn2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
3.8
-
DL-isocitrate
isoform ICD-1, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
4
-
NADP+
isoform ICD-1, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
19.6
-
DL-isocitrate
isoform ICD-2, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
37.4
-
NADP+
isoform ICD-2, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
both isoforms ICD-1, ICD-2
Mycobacterium tuberculosis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
5.5
-
isoform ICD-1, 30% of maximum activity, isoform ICD-2, 10% of maximum activity
Mycobacterium tuberculosis
1.1.1.42
9.5
-
isoform ICD-1, 90% of maximum activity, isoform ICD-2, less than 40% of maximum activity
Mycobacterium tuberculosis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
additional information
NAD+ may not replace NADP+
Mycobacterium tuberculosis
1.1.1.42
NADP+
required by both isoforms ICD-1 and ICD-2
Mycobacterium tuberculosis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.42
0.0046
-
NADPH
isoform ICD-2, pH 7.5, 25C
Mycobacterium tuberculosis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.42
NaCl
isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory
Mycobacterium tuberculosis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
additional information
NAD+ may not replace NADP+
Mycobacterium tuberculosis
1.1.1.42
NADP+
required by both isoforms ICD-1 and ICD-2
Mycobacterium tuberculosis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
NaCl
isoform ICD-2, increase in activity in up to 200 mM NaCl. Isoform ICD-1, no influence of NaCl. Above 200 mM, NaCl is inhibitory
Mycobacterium tuberculosis
1.1.1.42
NADPH
isoform ICD-2, competitive inhibition
Mycobacterium tuberculosis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.42
0.0046
-
NADPH
isoform ICD-2, pH 7.5, 25C
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.01
-
DL-isocitrate
isoform ICD-1, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
0.02
-
DL-isocitrate
isoform ICD-2, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
0.022
-
DL-isocitrate
isoform ICD-1, presence of Zn2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
0.125
-
NADP+
isoform ICD-1, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
19.6
-
NADP+
isoform ICD-2, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
both isoforms ICD-1, ICD-2, saturation at 10 mM
Mycobacterium tuberculosis
1.1.1.42
additional information
no acitivty of isoforms with Mn2+ or Ca2+
Mycobacterium tuberculosis
1.1.1.42
Zn2+
isoform ICD-1, can replace Mg2+, saturation at 10 mM
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
49000
-
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer; 4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1
Mycobacterium tuberculosis
1.1.1.42
86000
-
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer; and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2
Mycobacterium tuberculosis
1.1.1.42
100000
-
gel filtration, main peak, isoform ICD-1
Mycobacterium tuberculosis
1.1.1.42
180000
-
gel filtration, isoform ICD-2
Mycobacterium tuberculosis
1.1.1.42
200000
-
gel filtration, isoform ICD-1
Mycobacterium tuberculosis
1.1.1.42
320000
-
gel filtration, isoform ICD-2
Mycobacterium tuberculosis
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
isoform ICD-2, renaturation of protein inactivated for 30 min at 65C by slow cooling till 55C
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
-
668080
Mycobacterium tuberculosis
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
2 * 49000, isoform ICD-1, 2 * 86000, isoform ICD-2, SDS-PAGE. Besides dimers, isoform ICD-1 forms some tetramer, ICD-2 forms trimer
Mycobacterium tuberculosis
1.1.1.42
tetramer
4 * 49000, isoform ICD-1, SDS-PAGE. Main form is dimer for isoform ICD-1
Mycobacterium tuberculosis
1.1.1.42
trimer
and dimer, 3 * 86000, SDS-PAGE and chemical crosslinking, isoform ICD-2
Mycobacterium tuberculosis
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
20
40
isoform ICD-2
Mycobacterium tuberculosis
1.1.1.42
40
-
isoform ICD-1
Mycobacterium tuberculosis
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.1.1.42
60
-
isoform ICD-1, 40% residual activity, ICD-2, 5% residual activity
Mycobacterium tuberculosis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.42
65
-
30 min, isoform ICD-1, irreversible thermal inactivation, isoform ICD-2, renaturation of inactivated protein by slow cooling till 55C
Mycobacterium tuberculosis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.42
1.9
-
DL-isocitrate
isoform ICD-1, presence of Zn2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
3.8
-
DL-isocitrate
isoform ICD-1, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
4
-
NADP+
isoform ICD-1, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
19.6
-
DL-isocitrate
isoform ICD-2, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
1.1.1.42
37.4
-
NADP+
isoform ICD-2, presence of Mg2+, pH 7.5, 25C
Mycobacterium tuberculosis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
both isoforms ICD-1, ICD-2
Mycobacterium tuberculosis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
5.5
-
isoform ICD-1, 30% of maximum activity, isoform ICD-2, 10% of maximum activity
Mycobacterium tuberculosis
1.1.1.42
9.5
-
isoform ICD-1, 90% of maximum activity, isoform ICD-2, less than 40% of maximum activity
Mycobacterium tuberculosis