Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoshimune, K.; Hirayama, A.; Moriguchi, M.
    A metal ion as a cofactor attenuates substrate inhibition in the enzymatic production of a high concentration of D-glutamate using N-acyl-D-glutamate amidohydrolase (2005), Biotechnol. Lett., 27, 1325-1328.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.82 synthesis the enzyme is used for production of D-glutamate, Co2+ might be a useful additive for stabilization of the enzyme Achromobacter xylosoxidans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.82 overexpression of D-AGase in Escherichia coli Achromobacter xylosoxidans

General Stability

EC Number General Stability Organism
3.5.1.82 Co2+ stabilizes Achromobacter xylosoxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.82 N-acetyl-DL-glutamate substrate inhibition at high concentration, 94% inhibition at 1 M, the inhibition is reduced by Co2+ Achromobacter xylosoxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.82 Ba2+ stabilizes partially at 1 mM Achromobacter xylosoxidans
3.5.1.82 Co2+ stabilizes, optimal at 1 mM, no activation of enzymic activity Achromobacter xylosoxidans
3.5.1.82 Mg2+ stabilizes partially at 1 mM Achromobacter xylosoxidans
3.5.1.82 additional information effects on enzyme stability by metal ions, overview Achromobacter xylosoxidans
3.5.1.82 Zn2+ required, D-AGase is a zinc-metalloenzyme, stabilizes partially, no activation of enzymic activity Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.82 N-acetyl-D-glutamate + H2O Achromobacter xylosoxidans
-
 acetate + D-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.82 Achromobacter xylosoxidans
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.82 additional information
-
-
 Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.82 N-acetyl-D-glutamate + H2O
-
 Achromobacter xylosoxidans acetate + D-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.82 D-AGase
-
 Achromobacter xylosoxidans
3.5.1.82 N-acyl-D-glutamate amidohydrolase
-
 Achromobacter xylosoxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.82 30
-
 assay at Achromobacter xylosoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.82 7
-
 assay at Achromobacter xylosoxidans