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Literature summary extracted from

  • Kumar, R.S.; Suresh, C.G.; Pundle, A.; Prabhune, A.
    Evidence for the involvement of arginyl residue at the active site of penicillin G acylase from Kluyvera citrophila (2004), Biotechnol. Lett., 26, 1601-1606.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.11 2,3-Butanedione 50 mM, 78% loss of activity, benzylpenicillin or phenylacetate protects Kluyvera cryocrescens
3.5.1.11 Phenylglyoxal 20 mM, 82% loss of activity, benzylpenicillin or phenylacetate protects Kluyvera cryocrescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.11 0.016
-
 benzylpenicillin pH 7.5, 40°C Kluyvera cryocrescens
3.5.1.11 0.061
-
 benzylpenicillin pH 7.5, 40°C, presence of 50 mM 2,3-butanedione Kluyvera cryocrescens
3.5.1.11 0.065
-
 benzylpenicillin pH 7.5, 40°C, presence of 10 mM phenylglyoxal Kluyvera cryocrescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.11 24000
-
 x * 24000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE Kluyvera cryocrescens
3.5.1.11 62000
-
 x * 24000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE Kluyvera cryocrescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Kluyvera cryocrescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.11
-
 Kluyvera cryocrescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.11 18
-
 pH 7.5, 40°C Kluyvera cryocrescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 benzylpenicillin + H2O
-
 Kluyvera cryocrescens phenylacetic acid + 6-aminopenicillanate
-
 ?
3.5.1.11 penicillin G + H2O
-
 Kluyvera cryocrescens 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.11 ? x * 24000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE Kluyvera cryocrescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.11 21
-
 benzylpenicillin pH 7.5, 40°C, presence of 10 mM phenylglyoxal Kluyvera cryocrescens
3.5.1.11 27
-
 benzylpenicillin pH 7.5, 40°C, presence of 50 mM 2,3-butanedione Kluyvera cryocrescens
3.5.1.11 63
-
 benzylpenicillin pH 7.5, 40°C Kluyvera cryocrescens