Literature summary extracted from

Theppakorn, T.; Kanasawud, P.; Halling, P.J.
Activity of immobilized papain dehydrated by n-propanol in low-water media (2004), Biotechnol. Lett., 26, 133-136.

Application

EC Number	Application	Comment	Organism
3.4.22.2	biotechnology	immobilization of papain and dehydration by n-propanol in low-water media at pH 6.4 and 25°C in 150 mM sodium phosphate buffer, stability is increased by solid state cysteine, overview	Carica papaya

General Stability

EC Number	General Stability	Organism
3.4.22.2	solid state cysteine increases the stability of n-propanol dehydrated, immobilized enzyme in in low-water tert-butanol medium	Carica papaya

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.22.2	additional information	several buffers decrease the activity of the n-propanol dehydrated, immobilized enzyme in low-water tert-butanol medium, overview	Carica papaya

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.2	Carica papaya	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.4.22.2	0.059	-	activity of immobilized papain dehydrated by n-propanol in low-water tert-butanol media	Carica papaya

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.22.2	L-phenylalanine amide + H2O	-	Carica papaya	L-phenylalanine + NH3	-	?
3.4.22.2	N-benzyloxycarbonyl-L-glycine + H2O	-	Carica papaya	?	-	?

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.4.22.2	40	-	assay at	Carica papaya

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.4.22.2	6.4	-	assay at	Carica papaya

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Release 2023.1 (January 2023)